Vasopressin-2 receptor antagonist attenuates the ability of the lungs to clear edema in an experimental model

Julia Guetta, Geula Klorin, Ronit Tal, Gidon Berger, Reem Ismael-Badarneh, Bishara Bishara, Edmond Sabo, Zaid Abassi, Zaher S. Azzam

Research output: Contribution to journalArticlepeer-review


In the last two decades, the role of the alveolar active sodium transport was extensively studied and was found to play a crucial role in regulating alveolar fluid clearance (AFC), and thus in keeping the airspaces free of edema. The recent development of highly selective nonpeptide vasopressin-receptor antagonists gives us a rare chance to explore the role of vasopressin in the pathogenesis of lung edema. Therefore, the present study examined the involvement of vasopressin in modulating the ability of the lung to clear edema. Vasopressin enhanced the rate of lung edema clearance by 30% as compared with untreated control rats (from 0.49 ± 0.02 to 0.64 ± 0.02 ml/h), whereas V2 receptor antagonists significantly decreased the ability of the lung to clear water (from 0.64 ± 0.02 to 0.31 ± 0.06 ml/h; P < 0.0001). In contrast, V1 receptor antagonist did not change the rate of AFC. The administration of ouabain (a Na,K-ATPase inhibitor) and amiloride (a Na+ channel blocker) inhibited the stimulatory effects of vasopressin (from 0.64 6 0.02 to 0.22 ± 0.02 ml/h [P < 0.0001] and from 0.64 ± 0.017 to 0.23 ± 0.02 ml/h [P < 0.0001], respectively). Vasopressin significantly increased Na,K-ATPase protein abundance in the basolateral membranes of the alveolar epithelial cells via V2 receptor activation. We report a novel role of the vasopressin pathway in AFC. This observation indicates a beneficial role of vasopressin in AFC by up-regulating active sodium transport.

Original languageEnglish
Pages (from-to)583-588
Number of pages6
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Issue number5
StatePublished - Nov 2012
Externally publishedYes


  • Active sodium
  • Alveolar fluid clearance
  • Na,K-ATPase
  • V2 receptor
  • Vasopressin

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology


Dive into the research topics of 'Vasopressin-2 receptor antagonist attenuates the ability of the lungs to clear edema in an experimental model'. Together they form a unique fingerprint.

Cite this