Variation of sesquiterpene lactones in Lactuca aculeata natural populations from Israel, Jordan and Turkey

Alex Beharav; Roi Ben-David; Janusz Malarz; Anna Stojakowska; Klaudia Michalska; Ivana Doležalová; Aleš Lebeda; Wanda Kisiel

doi:10.1016/j.bse.2010.07.007

Variation of sesquiterpene lactones in Lactuca aculeata natural populations from Israel, Jordan and Turkey

Alex Beharav, Roi Ben-David, Janusz Malarz, Anna Stojakowska, Klaudia Michalska, Ivana Doležalová, Aleš Lebeda, Wanda Kisiel

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

A comparative phytochemical study of seven sesquiterpene lactones in natural populations of the wild lettuce Lactuca aculeata Boiss. (Asteraceae) was performed, based on 23 accessions derived from eight, two, and single localities from Israel, Jordan, and Turkey, respectively. The compounds were profiled and quantified in leaves and roots of the plants, grown from achenes (cypselas) in a greenhouse under controlled conditions, using reverse phase HPLC with on-line photodiode array detector. In the present study, L. aculeata was confirmed as a taxon strongly characterized by four dominant sesquiterpene lactones: 8-deoxylactucin, jacquinelin, crepidiaside B and lactuside A. An analysis of quantitative results of these four constituents led to the following conclusions: (i) the sesquiterpene lactone contents varied significantly, mainly within the populations; however, for two variables (crepidiaside B and lactuside A in root samples) the contents varied mostly between populations, (ii) these differences are likely to be genetically controlled since all accessions were grown under standardized glasshouse conditions. Accessions containing high levels of some sesquiterpene lactones were found in several populations. These traits should be conserved in situ and ex situ and utilized in further research and lettuce improvement.

Original language	English
Pages (from-to)	602-611
Number of pages	10
Journal	Biochemical Systematics and Ecology
Volume	38
Issue number	4
DOIs	https://doi.org/10.1016/j.bse.2010.07.007
State	Published - Aug 2010

Bibliographical note

Funding Information:
A. Beharav thanks the Research Authority, University of Haifa, Israel, for financial support for the unique germplasm collection used in this study. We also thank E. Nevo, Institute of Evolution, University of Haifa, for part of the germplasm used. The financial support of the Ministry of Science and Higher Education, Poland (statutory activity founding), is gratefully acknowledged. The research was also supported by grant MSM 6198959215 ( Ministry of Education, Youth and Sports of the Czech Republic ).

Keywords

Lactucin
Middle East
Natural populations
Plant germplasm
Secondary metabolite diversity
Wild lettuce

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Biochemistry

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10.1016/j.bse.2010.07.007

Cite this

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title = "Variation of sesquiterpene lactones in Lactuca aculeata natural populations from Israel, Jordan and Turkey",

abstract = "A comparative phytochemical study of seven sesquiterpene lactones in natural populations of the wild lettuce Lactuca aculeata Boiss. (Asteraceae) was performed, based on 23 accessions derived from eight, two, and single localities from Israel, Jordan, and Turkey, respectively. The compounds were profiled and quantified in leaves and roots of the plants, grown from achenes (cypselas) in a greenhouse under controlled conditions, using reverse phase HPLC with on-line photodiode array detector. In the present study, L. aculeata was confirmed as a taxon strongly characterized by four dominant sesquiterpene lactones: 8-deoxylactucin, jacquinelin, crepidiaside B and lactuside A. An analysis of quantitative results of these four constituents led to the following conclusions: (i) the sesquiterpene lactone contents varied significantly, mainly within the populations; however, for two variables (crepidiaside B and lactuside A in root samples) the contents varied mostly between populations, (ii) these differences are likely to be genetically controlled since all accessions were grown under standardized glasshouse conditions. Accessions containing high levels of some sesquiterpene lactones were found in several populations. These traits should be conserved in situ and ex situ and utilized in further research and lettuce improvement.",

keywords = "Lactucin, Middle East, Natural populations, Plant germplasm, Secondary metabolite diversity, Wild lettuce",

author = "Alex Beharav and Roi Ben-David and Janusz Malarz and Anna Stojakowska and Klaudia Michalska and Ivana Dole{\v z}alov{\'a} and Ale{\v s} Lebeda and Wanda Kisiel",

note = "Funding Information: A. Beharav thanks the Research Authority, University of Haifa, Israel, for financial support for the unique germplasm collection used in this study. We also thank E. Nevo, Institute of Evolution, University of Haifa, for part of the germplasm used. The financial support of the Ministry of Science and Higher Education, Poland (statutory activity founding), is gratefully acknowledged. The research was also supported by grant MSM 6198959215 ( Ministry of Education, Youth and Sports of the Czech Republic ).",

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doi = "10.1016/j.bse.2010.07.007",

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T1 - Variation of sesquiterpene lactones in Lactuca aculeata natural populations from Israel, Jordan and Turkey

AU - Beharav, Alex

AU - Ben-David, Roi

AU - Malarz, Janusz

AU - Stojakowska, Anna

AU - Michalska, Klaudia

AU - Doležalová, Ivana

AU - Lebeda, Aleš

AU - Kisiel, Wanda

N1 - Funding Information: A. Beharav thanks the Research Authority, University of Haifa, Israel, for financial support for the unique germplasm collection used in this study. We also thank E. Nevo, Institute of Evolution, University of Haifa, for part of the germplasm used. The financial support of the Ministry of Science and Higher Education, Poland (statutory activity founding), is gratefully acknowledged. The research was also supported by grant MSM 6198959215 ( Ministry of Education, Youth and Sports of the Czech Republic ).

PY - 2010/8

Y1 - 2010/8

N2 - A comparative phytochemical study of seven sesquiterpene lactones in natural populations of the wild lettuce Lactuca aculeata Boiss. (Asteraceae) was performed, based on 23 accessions derived from eight, two, and single localities from Israel, Jordan, and Turkey, respectively. The compounds were profiled and quantified in leaves and roots of the plants, grown from achenes (cypselas) in a greenhouse under controlled conditions, using reverse phase HPLC with on-line photodiode array detector. In the present study, L. aculeata was confirmed as a taxon strongly characterized by four dominant sesquiterpene lactones: 8-deoxylactucin, jacquinelin, crepidiaside B and lactuside A. An analysis of quantitative results of these four constituents led to the following conclusions: (i) the sesquiterpene lactone contents varied significantly, mainly within the populations; however, for two variables (crepidiaside B and lactuside A in root samples) the contents varied mostly between populations, (ii) these differences are likely to be genetically controlled since all accessions were grown under standardized glasshouse conditions. Accessions containing high levels of some sesquiterpene lactones were found in several populations. These traits should be conserved in situ and ex situ and utilized in further research and lettuce improvement.

AB - A comparative phytochemical study of seven sesquiterpene lactones in natural populations of the wild lettuce Lactuca aculeata Boiss. (Asteraceae) was performed, based on 23 accessions derived from eight, two, and single localities from Israel, Jordan, and Turkey, respectively. The compounds were profiled and quantified in leaves and roots of the plants, grown from achenes (cypselas) in a greenhouse under controlled conditions, using reverse phase HPLC with on-line photodiode array detector. In the present study, L. aculeata was confirmed as a taxon strongly characterized by four dominant sesquiterpene lactones: 8-deoxylactucin, jacquinelin, crepidiaside B and lactuside A. An analysis of quantitative results of these four constituents led to the following conclusions: (i) the sesquiterpene lactone contents varied significantly, mainly within the populations; however, for two variables (crepidiaside B and lactuside A in root samples) the contents varied mostly between populations, (ii) these differences are likely to be genetically controlled since all accessions were grown under standardized glasshouse conditions. Accessions containing high levels of some sesquiterpene lactones were found in several populations. These traits should be conserved in situ and ex situ and utilized in further research and lettuce improvement.

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KW - Middle East

KW - Natural populations

KW - Plant germplasm

KW - Secondary metabolite diversity

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M3 - Article

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SN - 0305-1978

VL - 38

SP - 602

EP - 611

JO - Biochemical Systematics and Ecology

JF - Biochemical Systematics and Ecology

IS - 4

ER -

Variation of sesquiterpene lactones in Lactuca aculeata natural populations from Israel, Jordan and Turkey

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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