Unidirectional Notch signaling depends on continuous cleavage of Delta

Amir Sapir, Efrat Assa-Kunik, Rachel Tsruya, Eyal Schejter, Ben Zion Shilo

Research output: Contribution to journalArticlepeer-review


Unidirectional signaling from cells expressing Delta (Dl) to cells expressing Notch is a key feature of many developmental processes. We demonstrate that the Drosophila ADAM metalloprotease Kuzbanian-like (Kul) plays a key role in promoting this asymmetry. Kul cleaves Dl efficiently both in cell culture and in flies, and has previously been shown not to be necessary for Notch processing during signaling. In the absence of Kul in the developing wing, the level of Dl in cells that normally receive the signal is elevated, and subsequent alterations in the directionality of Notch signaling lead to prominent phenotypic defects. Proteolytic cleavage of Dl by Kul represents a general mechanism for refining and maintaining the asymmetric distribution of Dl, in cases where transcriptional repression of Dl expression does not suffice to eliminate Dl protein.

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalDevelopment (Cambridge)
Issue number1
StatePublished - Jan 2005
Externally publishedYes


  • ADAM metalloproteases
  • Delta
  • Drosophila
  • Kuzbanian-like
  • Notch
  • Serrate
  • Wing development

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


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