Unexpected expression of carbonic anhydrase I and selenium-binding protein as the only major non-heme proteins in erythrocytes of the subterranean mole rat (Spalax ehrenbergi)

Hong Yang, Eviatar Nevo, Richard E. Tashian

Research output: Contribution to journalArticlepeer-review

Abstract

Chromatographic separation of the non-heme proteins from the erythrocytes of the subterranean mole rat belonging to the superspecies Spalax ehrenbergi from Israel revealed two major peaks. On sequence analyses, the larger peak corresponded to a 56 kDa selenium-binding protein (SeBP) previously characterized from mouse and human liver, and the second peak to the low-activity carbonic anhydrase (CA) isozyme, CA I. There was no evidence of the high-activity CA II isozyme normally found in the red cells of all amniotes tested to date. Thus, the mole rat appears to be the first mammalian species to express both a SeBP and the low-activity CA I isozyme, as the major non-heme proteins in its red blood cells. It is possible that the absence of the high-activity CA II isozyme may be advantageous to the mole rat in adapting to the low O2 and high CO2 environment of its underground burrows. It is also likely that the 56 kDa SeBP may play an important adaptive role in the physiology of the red cell.

Original languageEnglish
Pages (from-to)343-347
Number of pages5
JournalFEBS Letters
Volume430
Issue number3
DOIs
StatePublished - 3 Jul 1998

Bibliographical note

Funding Information:
We thank Ya-shiou Yu and Charles Mitchell (University of Michigan) for technical assistance, and Drs. David Hewett-Emmett (University of Texas) and Erik Swenson (University of Washington, Seattle) for helpful advice and discussions. The research is supported by NIH Grant GM-24681 (R.E.T.), and the Israeli Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution (E.N.).

Keywords

  • Carbonic anhydrases I and II
  • Hypercapnia
  • Hypoxia
  • Mote rat erythrocyte
  • Respiratory acidosis
  • Selenium-binding protein

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Cell Biology

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