TY - JOUR
T1 - Two complementary NLRs from wild emmer wheat confer powdery mildew resistance
AU - Yang, Zuhuan
AU - Liu, Nannan
AU - Xie, Xiaoming
AU - Wei, Wenxin
AU - Bai, Yuhan
AU - Sun, Junna
AU - Pan, Wei
AU - Yang, Jiatian
AU - Wang, Weidong
AU - Xie, Xiaodong
AU - Saqlain, Muhammad
AU - Kang, Houyang
AU - Li, Baoyun
AU - Hu, Zhaorong
AU - Gou, Jinying
AU - Guo, Weilong
AU - Song, Susheng
AU - Ma, Jun
AU - Fahima, Tzion
AU - Sun, Qixin
AU - Qiu, Lina
AU - Li, Yinghui
AU - Xie, Chaojie
N1 - Publisher Copyright:
© The Author(s) 2025.
PY - 2025/12
Y1 - 2025/12
N2 - Powdery mildew is a devastating disease that affects wheat yield and quality globally. Here, we identify a powdery mildew resistance locus MlIW39 from wild emmer wheat through map-based cloning, mutagenesis, and stable genetic transformation. Unlike many other cloned Pm genes, the MlIW39-mediated resistance is conferred by the combined effect of two complementary nucleotide-binding and leucine-rich repeat (NLR) genes, encoding a canonical coiled-coil (CC) type NLR protein (MlIW39-R1) and an atypical NLR protein (MlIW39-R2) with an unknown domain (CC-like), respectively. Overexpression of the NLR pair induces cell death in Nicotiana benthamiana, whereas MlIW39-R1 or MlIW39-R2 alone does not. The MlIW39-R1 and MlIW39-R2 proteins physically interact with each other. MlIW39-R1 and MlIW39-R2 likely originate independently and become neighborly located during evolution. Our findings shed light on the significance of NLR pairs in plant immunity and can facilitate wheat disease-resistance breeding using the developed MlIW39 introgression lines and functional marker.
AB - Powdery mildew is a devastating disease that affects wheat yield and quality globally. Here, we identify a powdery mildew resistance locus MlIW39 from wild emmer wheat through map-based cloning, mutagenesis, and stable genetic transformation. Unlike many other cloned Pm genes, the MlIW39-mediated resistance is conferred by the combined effect of two complementary nucleotide-binding and leucine-rich repeat (NLR) genes, encoding a canonical coiled-coil (CC) type NLR protein (MlIW39-R1) and an atypical NLR protein (MlIW39-R2) with an unknown domain (CC-like), respectively. Overexpression of the NLR pair induces cell death in Nicotiana benthamiana, whereas MlIW39-R1 or MlIW39-R2 alone does not. The MlIW39-R1 and MlIW39-R2 proteins physically interact with each other. MlIW39-R1 and MlIW39-R2 likely originate independently and become neighborly located during evolution. Our findings shed light on the significance of NLR pairs in plant immunity and can facilitate wheat disease-resistance breeding using the developed MlIW39 introgression lines and functional marker.
UR - https://www.scopus.com/pages/publications/105018397675
U2 - 10.1038/s41467-025-64052-3
DO - 10.1038/s41467-025-64052-3
M3 - Article
C2 - 41073396
AN - SCOPUS:105018397675
SN - 2041-1723
VL - 16
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 9041
ER -