Trials and tribulations with VH replacement

Wenzhao Meng; Sahana Jayaraman; Bochao Zhang; Gregory W. Schwartz; Robert D. Daber; Uri Hershberg; Alfred L. Garfall; Christopher S. Carlson; Eline T. Luning Prak

doi:10.3389/fimmu.2014.00010

Trials and tribulations with VH replacement

Wenzhao Meng, Sahana Jayaraman, Bochao Zhang, Gregory W. Schwartz, Robert D. Daber, Uri Hershberg, Alfred L. Garfall, Christopher S. Carlson, Eline T. Luning Prak

Research output: Contribution to journal › Article › peer-review

Abstract

VH replacement (VHR) is a type of antibody gene rearrangement in which an upstream heavy chain variable gene segment (VH) invades a pre-existing rearrangement (VDJ). In this Hypothesis and Theory article, we begin by reviewing the mechanism of VHR, its developmental timing and its potential biological consequences. Then we explore the hypothesis that specific sequence motifs called footprints reflect VHR versus other processes. We provide a compilation of footprint sequences from different regions of the antibody heavy chain, and include data from the literature and from a high throughput sequencing experiment to evaluate the significance of footprint sequences. We conclude by discussing the difficulties of attributing footprints to VHR.

Original language	English
Article number	Article 10
Journal	Frontiers in Immunology
Volume	5
Issue number	JAN
DOIs	https://doi.org/10.3389/fimmu.2014.00010
State	Published - 2014
Externally published	Yes

Keywords

And JH gene segments
DH
Receptor editing
V(D)J recombination
VH
VH replacement

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.3389/fimmu.2014.00010

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title = "Trials and tribulations with VH replacement",

abstract = "VH replacement (VHR) is a type of antibody gene rearrangement in which an upstream heavy chain variable gene segment (VH) invades a pre-existing rearrangement (VDJ). In this Hypothesis and Theory article, we begin by reviewing the mechanism of VHR, its developmental timing and its potential biological consequences. Then we explore the hypothesis that specific sequence motifs called footprints reflect VHR versus other processes. We provide a compilation of footprint sequences from different regions of the antibody heavy chain, and include data from the literature and from a high throughput sequencing experiment to evaluate the significance of footprint sequences. We conclude by discussing the difficulties of attributing footprints to VHR.",

keywords = "And JH gene segments, DH, Receptor editing, V(D)J recombination, VH, VH replacement",

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T1 - Trials and tribulations with VH replacement

AU - Meng, Wenzhao

AU - Jayaraman, Sahana

AU - Zhang, Bochao

AU - Schwartz, Gregory W.

AU - Daber, Robert D.

AU - Hershberg, Uri

AU - Garfall, Alfred L.

AU - Carlson, Christopher S.

AU - Luning Prak, Eline T.

PY - 2014

Y1 - 2014

N2 - VH replacement (VHR) is a type of antibody gene rearrangement in which an upstream heavy chain variable gene segment (VH) invades a pre-existing rearrangement (VDJ). In this Hypothesis and Theory article, we begin by reviewing the mechanism of VHR, its developmental timing and its potential biological consequences. Then we explore the hypothesis that specific sequence motifs called footprints reflect VHR versus other processes. We provide a compilation of footprint sequences from different regions of the antibody heavy chain, and include data from the literature and from a high throughput sequencing experiment to evaluate the significance of footprint sequences. We conclude by discussing the difficulties of attributing footprints to VHR.

AB - VH replacement (VHR) is a type of antibody gene rearrangement in which an upstream heavy chain variable gene segment (VH) invades a pre-existing rearrangement (VDJ). In this Hypothesis and Theory article, we begin by reviewing the mechanism of VHR, its developmental timing and its potential biological consequences. Then we explore the hypothesis that specific sequence motifs called footprints reflect VHR versus other processes. We provide a compilation of footprint sequences from different regions of the antibody heavy chain, and include data from the literature and from a high throughput sequencing experiment to evaluate the significance of footprint sequences. We conclude by discussing the difficulties of attributing footprints to VHR.

KW - And JH gene segments

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KW - Receptor editing

KW - V(D)J recombination

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KW - VH replacement

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JO - Frontiers in Immunology

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Trials and tribulations with VH replacement

Abstract

Keywords

ASJC Scopus subject areas

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