Transfer Function Restoration in 3D Electron Microscopy via Iterative Data Refinement

C. O.S. Sorzano, José Jesús Fernández, R. Marabini, Gabor T. Herman, Yair Censor, J. M. Carazo

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Three-dimensional electron microscopy (3D-EM) aims at obtaining structural information of macromolecular complexes within a typical resolution range of between 2 to 0.3 nm from the projection images produced by an electron microscope. As any other imaging device, the electron microscope introduces a transfer function (called in this field Contrast Transfer Function, CTF) into the image acquisition process which modulates the different frequencies of the projection signal. Thus, the 3D reconstructions performed with these CTF-affected projections is also affected by an implicit 3D transfer function. Depending on the preparation procedure, the effect of this CTF is quite dramatic limiting severely the achievable resolution. In this work we make use of the Iterative Data Refinement technique to obtain CTF-free reconstructions.
It is shown that the approach can be successfully applied to noiseless as well as to noisy data.
Original languageEnglish
Title of host publicationProceedings of The Sixth International Meeting on Fully Three-Dimensional Image Reconstruction in Radiology and Nuclear Medicine
Place of PublicationPacific Grove, CA
Number of pages4
StatePublished - 5 Apr 2001


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