Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling

Kai Zhou; Paige Fleet; Eviatar Nevo; Xinquan Zhang; Genlou Sun

doi:10.1038/s41598-017-08391-2

Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling

Kai Zhou, Paige Fleet, Eviatar Nevo, Xinquan Zhang, Genlou Sun

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Colchicine was commonly used to artificially double chromosomes while the transcriptome changes in colchicine treated plants has rarely been characterized. To understand the molecular mechanism of colchicine on chromosome doubling, we characterized transcriptome data of diploid orchardgrass root after colchicine treatment. Our results showed that 3381 of differentially expressed genes (DEGs) were mainly affected by water stress, 1258 DEGs that were expressed significantly in sample DacR5tr but not in DacR5ck were considered to be mainly affected by colchicine and combination of water and colchicine. These DEGs mainly regulated by colchicine were enriched to gene ontology (GO) accessions of cation binding, catalytic activity, membrane and transporter activity, and enriched to Kyoto Encyclopedia of Genes and Genome (KEGG) pathways of phenylpropanoid biosynthesis, phenylalanine metabolism, plant hormone signal transduction and starch and sucrose metabolism. Genes related to microtubule, spindle, chromosomal kinetochore, vesicle, cellulose and processes of cytoplasm movement, chromatid segregation, membrane and cell wall development were inhibited by colchicine. Our results revealed that colchicine restrained the microtubules and inhibited gene expression of cytokinesis, which might slow down the cell activity, delay the cell into anaerobic respiration, resulting in apoptosis at late stage, and relieving of waterlogging.

Original language	English
Article number	8503
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-08391-2
State	Published - 1 Dec 2017

Bibliographical note

Funding Information:
This work was supported by the Natural Sciences and Engineering Research Council of Canada (grant number DDG-2015-00039, RGPIN-2017-05817) and a Senate Research Grant at Saint Mary’s University to GS, the earmarked fund for Modern Agro-industry Technology Research System (No. CARS-35-05) and the National Natural Science Foundation of China (NO NSFC 31372363) to XQZ. This research was conducted at the Biology Department of Saint Mary’s University, Canada. Kai Zhou was a visiting PhD student from Sichuan Agricultural University.

Funding Information:
This work was supported by the Natural Sciences and Engineering Research Council of Canada (grant number DDG-2015-00039, RGPIN-2017-05817) and a Senate Research Grant at Saint Mary's University to GS, the earmarked fund for Modern Agro-industry Technology Research System (No. CARS-35-05) and the National Natural Science Foundation of China (NO NSFC 31372363) to XQZ. This research was conducted at the Biology Department of Saint Mary's University, Canada. Kai Zhou was a visiting PhD student from Sichuan Agricultural University.

Publisher Copyright:
© 2017 The Author(s).

ASJC Scopus subject areas

General

Access to Document

10.1038/s41598-017-08391-2

Cite this

@article{864dba395be04dddac00f11d22bf8d01,

title = "Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling",

abstract = "Colchicine was commonly used to artificially double chromosomes while the transcriptome changes in colchicine treated plants has rarely been characterized. To understand the molecular mechanism of colchicine on chromosome doubling, we characterized transcriptome data of diploid orchardgrass root after colchicine treatment. Our results showed that 3381 of differentially expressed genes (DEGs) were mainly affected by water stress, 1258 DEGs that were expressed significantly in sample DacR5tr but not in DacR5ck were considered to be mainly affected by colchicine and combination of water and colchicine. These DEGs mainly regulated by colchicine were enriched to gene ontology (GO) accessions of cation binding, catalytic activity, membrane and transporter activity, and enriched to Kyoto Encyclopedia of Genes and Genome (KEGG) pathways of phenylpropanoid biosynthesis, phenylalanine metabolism, plant hormone signal transduction and starch and sucrose metabolism. Genes related to microtubule, spindle, chromosomal kinetochore, vesicle, cellulose and processes of cytoplasm movement, chromatid segregation, membrane and cell wall development were inhibited by colchicine. Our results revealed that colchicine restrained the microtubules and inhibited gene expression of cytokinesis, which might slow down the cell activity, delay the cell into anaerobic respiration, resulting in apoptosis at late stage, and relieving of waterlogging.",

author = "Kai Zhou and Paige Fleet and Eviatar Nevo and Xinquan Zhang and Genlou Sun",

note = "Funding Information: This work was supported by the Natural Sciences and Engineering Research Council of Canada (grant number DDG-2015-00039, RGPIN-2017-05817) and a Senate Research Grant at Saint Mary{\textquoteright}s University to GS, the earmarked fund for Modern Agro-industry Technology Research System (No. CARS-35-05) and the National Natural Science Foundation of China (NO NSFC 31372363) to XQZ. This research was conducted at the Biology Department of Saint Mary{\textquoteright}s University, Canada. Kai Zhou was a visiting PhD student from Sichuan Agricultural University. Funding Information: This work was supported by the Natural Sciences and Engineering Research Council of Canada (grant number DDG-2015-00039, RGPIN-2017-05817) and a Senate Research Grant at Saint Mary's University to GS, the earmarked fund for Modern Agro-industry Technology Research System (No. CARS-35-05) and the National Natural Science Foundation of China (NO NSFC 31372363) to XQZ. This research was conducted at the Biology Department of Saint Mary's University, Canada. Kai Zhou was a visiting PhD student from Sichuan Agricultural University. Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = dec,

day = "1",

doi = "10.1038/s41598-017-08391-2",

language = "English",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling

AU - Zhou, Kai

AU - Fleet, Paige

AU - Nevo, Eviatar

AU - Zhang, Xinquan

AU - Sun, Genlou

N1 - Funding Information: This work was supported by the Natural Sciences and Engineering Research Council of Canada (grant number DDG-2015-00039, RGPIN-2017-05817) and a Senate Research Grant at Saint Mary’s University to GS, the earmarked fund for Modern Agro-industry Technology Research System (No. CARS-35-05) and the National Natural Science Foundation of China (NO NSFC 31372363) to XQZ. This research was conducted at the Biology Department of Saint Mary’s University, Canada. Kai Zhou was a visiting PhD student from Sichuan Agricultural University. Funding Information: This work was supported by the Natural Sciences and Engineering Research Council of Canada (grant number DDG-2015-00039, RGPIN-2017-05817) and a Senate Research Grant at Saint Mary's University to GS, the earmarked fund for Modern Agro-industry Technology Research System (No. CARS-35-05) and the National Natural Science Foundation of China (NO NSFC 31372363) to XQZ. This research was conducted at the Biology Department of Saint Mary's University, Canada. Kai Zhou was a visiting PhD student from Sichuan Agricultural University. Publisher Copyright: © 2017 The Author(s).

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Colchicine was commonly used to artificially double chromosomes while the transcriptome changes in colchicine treated plants has rarely been characterized. To understand the molecular mechanism of colchicine on chromosome doubling, we characterized transcriptome data of diploid orchardgrass root after colchicine treatment. Our results showed that 3381 of differentially expressed genes (DEGs) were mainly affected by water stress, 1258 DEGs that were expressed significantly in sample DacR5tr but not in DacR5ck were considered to be mainly affected by colchicine and combination of water and colchicine. These DEGs mainly regulated by colchicine were enriched to gene ontology (GO) accessions of cation binding, catalytic activity, membrane and transporter activity, and enriched to Kyoto Encyclopedia of Genes and Genome (KEGG) pathways of phenylpropanoid biosynthesis, phenylalanine metabolism, plant hormone signal transduction and starch and sucrose metabolism. Genes related to microtubule, spindle, chromosomal kinetochore, vesicle, cellulose and processes of cytoplasm movement, chromatid segregation, membrane and cell wall development were inhibited by colchicine. Our results revealed that colchicine restrained the microtubules and inhibited gene expression of cytokinesis, which might slow down the cell activity, delay the cell into anaerobic respiration, resulting in apoptosis at late stage, and relieving of waterlogging.

AB - Colchicine was commonly used to artificially double chromosomes while the transcriptome changes in colchicine treated plants has rarely been characterized. To understand the molecular mechanism of colchicine on chromosome doubling, we characterized transcriptome data of diploid orchardgrass root after colchicine treatment. Our results showed that 3381 of differentially expressed genes (DEGs) were mainly affected by water stress, 1258 DEGs that were expressed significantly in sample DacR5tr but not in DacR5ck were considered to be mainly affected by colchicine and combination of water and colchicine. These DEGs mainly regulated by colchicine were enriched to gene ontology (GO) accessions of cation binding, catalytic activity, membrane and transporter activity, and enriched to Kyoto Encyclopedia of Genes and Genome (KEGG) pathways of phenylpropanoid biosynthesis, phenylalanine metabolism, plant hormone signal transduction and starch and sucrose metabolism. Genes related to microtubule, spindle, chromosomal kinetochore, vesicle, cellulose and processes of cytoplasm movement, chromatid segregation, membrane and cell wall development were inhibited by colchicine. Our results revealed that colchicine restrained the microtubules and inhibited gene expression of cytokinesis, which might slow down the cell activity, delay the cell into anaerobic respiration, resulting in apoptosis at late stage, and relieving of waterlogging.

UR - http://www.scopus.com/inward/record.url?scp=85027856242&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-08391-2

DO - 10.1038/s41598-017-08391-2

M3 - Article

C2 - 28819246

AN - SCOPUS:85027856242

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 8503

ER -

Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this