The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells

Mikheil D. Asatiani; Solomon P. Wasser; Eviatar Nevo; Nili Ruimi; Jamal Mahajna; Abraham Z. Reznick

doi:10.1615/IntJMedMushr.v13.i1.30

The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells

Mikheil D. Asatiani, Solomon P. Wasser, Eviatar Nevo, Nili Ruimi, Jamal Mahajna, Abraham Z. Reznick

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-κB) transcription factor. The IC ₅₀ value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 μg/mL, and the ₅₀ value of C. comatus ethyl acetate extract was only 32 μg/ mL. Our results also showed that both extracts significantly affected IκBα phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-κB pathway inhibitor, and seemed to be the most active inhibitor of H ₂O ₂-dependent IκBα phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-κB function and also possibly acts as an antitumor agent.

Original language	English
Pages (from-to)	19-25
Number of pages	7
Journal	International Journal of Medicinal Mushrooms
Volume	13
Issue number	1
DOIs	https://doi.org/10.1615/IntJMedMushr.v13.i1.30
State	Published - 2011

Bibliographical note

Funding Information:
Received January 14, 1986. Accepted October 2, 1986. Address requests for reprints to: J. H. Szurszewski, Ph.D., Department of Physiology and Biophysics, Mayo Foundation, Rochester, Minnesota 55905. Parts of this study were previously published in abstract form (GASTROENTEROLOGY 1982;82:1080). This work was supported by a grant from the National Institutes of Health (AM 17632). The authors thank Dr. David Donald for guidance and assistance in performing the surgical procedures, Phil Schmalz for assistance in the operating procedures and for stastical analysis of the data, and Jan Applequist for patiently typing the manuscript. © 1987 by the American Gastroenterological Association

Keywords

Coprinus comatus substances
IKK complex
IκBα phosphorylation
Medicinal mushrooms
NF-κB activation pathway

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Pharmacology
Drug Discovery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Asatiani, M. D., Wasser, S. P., Nevo, E., Ruimi, N., Mahajna, J., & Reznick, A. Z. (2011). The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells. International Journal of Medicinal Mushrooms, 13(1), 19-25. https://doi.org/10.1615/IntJMedMushr.v13.i1.30

Asatiani, Mikheil D. ; Wasser, Solomon P. ; Nevo, Eviatar et al. / The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells. In: International Journal of Medicinal Mushrooms. 2011 ; Vol. 13, No. 1. pp. 19-25.

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title = "The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells",

abstract = "Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-κB) transcription factor. The IC 50 value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 μg/mL, and the 50 value of C. comatus ethyl acetate extract was only 32 μg/ mL. Our results also showed that both extracts significantly affected IκBα phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-κB pathway inhibitor, and seemed to be the most active inhibitor of H 2O 2-dependent IκBα phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-κB function and also possibly acts as an antitumor agent.",

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author = "Asatiani, {Mikheil D.} and Wasser, {Solomon P.} and Eviatar Nevo and Nili Ruimi and Jamal Mahajna and Reznick, {Abraham Z.}",

note = "Funding Information: Received January 14, 1986. Accepted October 2, 1986. Address requests for reprints to: J. H. Szurszewski, Ph.D., Department of Physiology and Biophysics, Mayo Foundation, Rochester, Minnesota 55905. Parts of this study were previously published in abstract form (GASTROENTEROLOGY 1982;82:1080). This work was supported by a grant from the National Institutes of Health (AM 17632). The authors thank Dr. David Donald for guidance and assistance in performing the surgical procedures, Phil Schmalz for assistance in the operating procedures and for stastical analysis of the data, and Jan Applequist for patiently typing the manuscript. {\textcopyright} 1987 by the American Gastroenterological Association",

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Asatiani, MD, Wasser, SP , Nevo, E, Ruimi, N, Mahajna, J & Reznick, AZ 2011, 'The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells', International Journal of Medicinal Mushrooms, vol. 13, no. 1, pp. 19-25. https://doi.org/10.1615/IntJMedMushr.v13.i1.30

The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells. / Asatiani, Mikheil D.; Wasser, Solomon P.; Nevo, Eviatar et al.
In: International Journal of Medicinal Mushrooms, Vol. 13, No. 1, 2011, p. 19-25.

Research output: Contribution to journal › Article › peer-review

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T1 - The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells

AU - Asatiani, Mikheil D.

AU - Wasser, Solomon P.

AU - Nevo, Eviatar

AU - Ruimi, Nili

AU - Mahajna, Jamal

AU - Reznick, Abraham Z.

N1 - Funding Information: Received January 14, 1986. Accepted October 2, 1986. Address requests for reprints to: J. H. Szurszewski, Ph.D., Department of Physiology and Biophysics, Mayo Foundation, Rochester, Minnesota 55905. Parts of this study were previously published in abstract form (GASTROENTEROLOGY 1982;82:1080). This work was supported by a grant from the National Institutes of Health (AM 17632). The authors thank Dr. David Donald for guidance and assistance in performing the surgical procedures, Phil Schmalz for assistance in the operating procedures and for stastical analysis of the data, and Jan Applequist for patiently typing the manuscript. © 1987 by the American Gastroenterological Association

PY - 2011

Y1 - 2011

N2 - Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-κB) transcription factor. The IC 50 value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 μg/mL, and the 50 value of C. comatus ethyl acetate extract was only 32 μg/ mL. Our results also showed that both extracts significantly affected IκBα phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-κB pathway inhibitor, and seemed to be the most active inhibitor of H 2O 2-dependent IκBα phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-κB function and also possibly acts as an antitumor agent.

AB - Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-κB) transcription factor. The IC 50 value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 μg/mL, and the 50 value of C. comatus ethyl acetate extract was only 32 μg/ mL. Our results also showed that both extracts significantly affected IκBα phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-κB pathway inhibitor, and seemed to be the most active inhibitor of H 2O 2-dependent IκBα phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-κB function and also possibly acts as an antitumor agent.

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KW - IKK complex

KW - IκBα phosphorylation

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KW - NF-κB activation pathway

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Asatiani MD, Wasser SP , Nevo E, Ruimi N, Mahajna J, Reznick AZ. The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells. International Journal of Medicinal Mushrooms. 2011;13(1):19-25. doi: 10.1615/IntJMedMushr.v13.i1.30

The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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