The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull. Fr.) Pers. (Agaricomycetideae) substances interfere with H2o2 induction of the NF-κB pathway through inhibition of IκBα phosphorylation in MCF7 breast cancer cells

Mikheil D. Asatiani, Solomon P. Wasser, Eviatar Nevo, Nili Ruimi, Jamal Mahajna, Abraham Z. Reznick

Research output: Contribution to journalArticlepeer-review

Abstract

Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-κB) transcription factor. The IC 50 value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 μg/mL, and the 50 value of C. comatus ethyl acetate extract was only 32 μg/ mL. Our results also showed that both extracts significantly affected IκBα phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-κB pathway inhibitor, and seemed to be the most active inhibitor of H 2O 2-dependent IκBα phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-κB function and also possibly acts as an antitumor agent.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalInternational Journal of Medicinal Mushrooms
Volume13
Issue number1
DOIs
StatePublished - 2011

Keywords

  • Coprinus comatus substances
  • IκBα phosphorylation
  • IKK complex
  • Medicinal mushrooms
  • NF-κB activation pathway

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Pharmacology
  • Drug Discovery

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