The role of Drosophila TNF Eiger in developmental and damage-induced neuronal apoptosis

Jeny Shklover; Flonia Levy-Adam; Estee Kurant

doi:10.1016/j.febslet.2015.02.032

The role of Drosophila TNF Eiger in developmental and damage-induced neuronal apoptosis

Jeny Shklover, Flonia Levy-Adam, Estee Kurant

Research output: Contribution to journal › Article › peer-review

Abstract

Eiger, the sole Drosophila TNF-alpha homolog, causes ectopic apoptosis through JNK pathway activation. Yet, its role in developmental apoptosis remains unclear. eiger mutant flies are viable and fertile but display compromised elimination of oncogenic cells and extracellular bacteria. Here we show that Eiger, specifically expressed in embryonic neurons and glia, is not involved in developmental neuronal apoptosis or in apoptotic cell clearance. Instead, we provide evidence that Eiger is required for damage-induced apoptosis in the embryonic CNS through regulation of the pro-apoptotic gene hid independently of the JNK pathway. Our study thus reveals a new requirement for Eiger in eliminating damaged cells during development.

Original language	English
Pages (from-to)	871-879
Number of pages	9
Journal	FEBS Letters
Volume	589
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2015.02.032
State	Published - 2 Apr 2015
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank B. Jones, D. Bohmann, G.W. Davis, E. Arama, H. Steller, M. Miura, O. Schuldiner, A. Salzberg, Hybridoma bank and the Bloomington Stock Center for generously providing fly strains and antibodies. We thank Dr. Alexander Nevelsky and Egor Borzov from Department of Oncology, Rambam Medical Center (RMC) for their assistance in the X-ray experiments. We thank Z. Paroush and T. Schultheiss for comments on the manuscript and the Kurant laboratory members for constructive criticism and support. We also thank E. Suss-Toby at the Interdepartmental Bioimaging facility for excellent technical support. We gratefully acknowledge financial support from Rappaport Institute and from Allen and Jewell Prince Center for Neurodegenerative Disorders of the Brain.

Publisher Copyright:
© 2015 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. This is an open access article under the CC BY-NC-ND license.

Keywords

Apoptosis
CNS
Drosophila
Eiger
Embryo
hid

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2015.02.032

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abstract = "Eiger, the sole Drosophila TNF-alpha homolog, causes ectopic apoptosis through JNK pathway activation. Yet, its role in developmental apoptosis remains unclear. eiger mutant flies are viable and fertile but display compromised elimination of oncogenic cells and extracellular bacteria. Here we show that Eiger, specifically expressed in embryonic neurons and glia, is not involved in developmental neuronal apoptosis or in apoptotic cell clearance. Instead, we provide evidence that Eiger is required for damage-induced apoptosis in the embryonic CNS through regulation of the pro-apoptotic gene hid independently of the JNK pathway. Our study thus reveals a new requirement for Eiger in eliminating damaged cells during development.",

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The role of Drosophila TNF Eiger in developmental and damage-induced neuronal apoptosis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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