TY - JOUR
T1 - The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN
AU - Mazo-Molina, Carolina
AU - Mainiero, Samantha
AU - Hind, Sarah R.
AU - Kraus, Christine M.
AU - Vachev, Mishi
AU - Maviane-Macia, Felicia
AU - Lindeberg, Magdalen
AU - Saha, Surya
AU - Strickler, Susan R.
AU - Feder, Ari
AU - Giovannoni, James J.
AU - Smart, Christine D.
AU - Peeters, Nemo
AU - Martin, Gregory B.
N1 - Publisher Copyright:
© 2019 The American Phytopathological Society.
PY - 2019
Y1 - 2019
N2 - Race 1 strains of Pseudomonas syringae pv. tomato, which cause bacterial speck disease of tomato, are becoming increasingly common and no simply inherited genetic resistance to such strains is known. We discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 P. syringae pv. tomato strains by detecting the activity of type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades the RIN4 protein, thereby activating RPS2-mediated immunity. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 also detected the activity of AvrRpt2 homologs from diverse bacteria, including one in Ralstonia pseudosolanacearum. The genome sequence of S. lycopersicoides revealed no RPS2 homolog in the Ptr1 region. Ptr1 could play an important role in controlling bacterial speck disease and its future cloning may shed light on an example of convergent evolution for recognition of a widespread type III effector.
AB - Race 1 strains of Pseudomonas syringae pv. tomato, which cause bacterial speck disease of tomato, are becoming increasingly common and no simply inherited genetic resistance to such strains is known. We discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 P. syringae pv. tomato strains by detecting the activity of type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades the RIN4 protein, thereby activating RPS2-mediated immunity. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 also detected the activity of AvrRpt2 homologs from diverse bacteria, including one in Ralstonia pseudosolanacearum. The genome sequence of S. lycopersicoides revealed no RPS2 homolog in the Ptr1 region. Ptr1 could play an important role in controlling bacterial speck disease and its future cloning may shed light on an example of convergent evolution for recognition of a widespread type III effector.
KW - AvrRpt2
KW - Disease resistance
KW - Mr5
KW - NLR-triggered immunity
KW - RIN4
KW - RPS2
KW - Solanum lycopersicoides
KW - Solanum lycopersicum
UR - http://www.scopus.com/inward/record.url?scp=85064000785&partnerID=8YFLogxK
U2 - 10.1094/MPMI-01-19-0018-R
DO - 10.1094/MPMI-01-19-0018-R
M3 - Article
C2 - 30785360
AN - SCOPUS:85064000785
SN - 0894-0282
VL - 32
SP - 946
EP - 960
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 8
ER -