The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN

Carolina Mazo-Molina; Samantha Mainiero; Sarah R. Hind; Christine M. Kraus; Mishi Vachev; Felicia Maviane-Macia; Magdalen Lindeberg; Surya Saha; Susan R. Strickler; Ari Feder; James J. Giovannoni; Christine D. Smart; Nemo Peeters; Gregory B. Martin

doi:10.1094/MPMI-01-19-0018-R

The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN

Carolina Mazo-Molina, Samantha Mainiero, Sarah R. Hind, Christine M. Kraus, Mishi Vachev, Felicia Maviane-Macia, Magdalen Lindeberg, Surya Saha, Susan R. Strickler, Ari Feder, James J. Giovannoni, Christine D. Smart, Nemo Peeters, Gregory B. Martin

Research output: Contribution to journal › Article › peer-review

Abstract

Race 1 strains of Pseudomonas syringae pv. tomato, which cause bacterial speck disease of tomato, are becoming increasingly common and no simply inherited genetic resistance to such strains is known. We discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 P. syringae pv. tomato strains by detecting the activity of type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades the RIN4 protein, thereby activating RPS2-mediated immunity. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 also detected the activity of AvrRpt2 homologs from diverse bacteria, including one in Ralstonia pseudosolanacearum. The genome sequence of S. lycopersicoides revealed no RPS2 homolog in the Ptr1 region. Ptr1 could play an important role in controlling bacterial speck disease and its future cloning may shed light on an example of convergent evolution for recognition of a widespread type III effector.

Original language	English
Pages (from-to)	946-960
Number of pages	15
Journal	Molecular Plant-Microbe Interactions
Volume	32
Issue number	8
DOIs	https://doi.org/10.1094/MPMI-01-19-0018-R
State	Published - 2019
Externally published	Yes

Bibliographical note

Funding Information:
Funding: This work was supported by National Science Foundation (NSF) grants IOS-1546625 (to G. B. Martin) and IOS-0923312 (to J. J. Giovannoni), NSF Research Experience for Undergraduates DBI-1358843 (to M. Vachev), Laboratoire d’Excellence (LABEX), TULIP (ANR-10-LABX-41 to N. Peeters and F. Maviane-Macia), and Colciencias Depar-tamento Administrativo de Ciencia, Tecnología e Innovació grant 673 (to C. Mazo-Molina).

Publisher Copyright:
© 2019 The American Phytopathological Society.

Keywords

AvrRpt2
Disease resistance
Mr5
NLR-triggered immunity
RIN4
RPS2
Solanum lycopersicoides
Solanum lycopersicum

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science

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Mazo-Molina, C., Mainiero, S., Hind, S. R., Kraus, C. M., Vachev, M., Maviane-Macia, F., Lindeberg, M., Saha, S., Strickler, S. R., Feder, A., Giovannoni, J. J., Smart, C. D., Peeters, N., & Martin, G. B. (2019). The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN. Molecular Plant-Microbe Interactions, 32(8), 946-960. https://doi.org/10.1094/MPMI-01-19-0018-R

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title = "The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN",

abstract = "Race 1 strains of Pseudomonas syringae pv. tomato, which cause bacterial speck disease of tomato, are becoming increasingly common and no simply inherited genetic resistance to such strains is known. We discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 P. syringae pv. tomato strains by detecting the activity of type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades the RIN4 protein, thereby activating RPS2-mediated immunity. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 also detected the activity of AvrRpt2 homologs from diverse bacteria, including one in Ralstonia pseudosolanacearum. The genome sequence of S. lycopersicoides revealed no RPS2 homolog in the Ptr1 region. Ptr1 could play an important role in controlling bacterial speck disease and its future cloning may shed light on an example of convergent evolution for recognition of a widespread type III effector.",

keywords = "AvrRpt2, Disease resistance, Mr5, NLR-triggered immunity, RIN4, RPS2, Solanum lycopersicoides, Solanum lycopersicum",

author = "Carolina Mazo-Molina and Samantha Mainiero and Hind, {Sarah R.} and Kraus, {Christine M.} and Mishi Vachev and Felicia Maviane-Macia and Magdalen Lindeberg and Surya Saha and Strickler, {Susan R.} and Ari Feder and Giovannoni, {James J.} and Smart, {Christine D.} and Nemo Peeters and Martin, {Gregory B.}",

note = "Funding Information: Funding: This work was supported by National Science Foundation (NSF) grants IOS-1546625 (to G. B. Martin) and IOS-0923312 (to J. J. Giovannoni), NSF Research Experience for Undergraduates DBI-1358843 (to M. Vachev), Laboratoire d{\textquoteright}Excellence (LABEX), TULIP (ANR-10-LABX-41 to N. Peeters and F. Maviane-Macia), and Colciencias Depar-tamento Administrativo de Ciencia, Tecnolog{\'i}a e Innovaci{\'o} grant 673 (to C. Mazo-Molina). Publisher Copyright: {\textcopyright} 2019 The American Phytopathological Society.",

year = "2019",

doi = "10.1094/MPMI-01-19-0018-R",

language = "English",

volume = "32",

pages = "946--960",

journal = "Molecular Plant-Microbe Interactions",

issn = "0894-0282",

publisher = "American Phytopathological Society",

number = "8",

}

Mazo-Molina, C, Mainiero, S, Hind, SR, Kraus, CM, Vachev, M, Maviane-Macia, F, Lindeberg, M, Saha, S, Strickler, SR, Feder, A, Giovannoni, JJ, Smart, CD, Peeters, N & Martin, GB 2019, 'The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN', Molecular Plant-Microbe Interactions, vol. 32, no. 8, pp. 946-960. https://doi.org/10.1094/MPMI-01-19-0018-R

The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN. / Mazo-Molina, Carolina; Mainiero, Samantha; Hind, Sarah R. et al.
In: Molecular Plant-Microbe Interactions, Vol. 32, No. 8, 2019, p. 946-960.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN

AU - Mazo-Molina, Carolina

AU - Mainiero, Samantha

AU - Hind, Sarah R.

AU - Kraus, Christine M.

AU - Vachev, Mishi

AU - Maviane-Macia, Felicia

AU - Lindeberg, Magdalen

AU - Saha, Surya

AU - Strickler, Susan R.

AU - Feder, Ari

AU - Giovannoni, James J.

AU - Smart, Christine D.

AU - Peeters, Nemo

AU - Martin, Gregory B.

N1 - Funding Information: Funding: This work was supported by National Science Foundation (NSF) grants IOS-1546625 (to G. B. Martin) and IOS-0923312 (to J. J. Giovannoni), NSF Research Experience for Undergraduates DBI-1358843 (to M. Vachev), Laboratoire d’Excellence (LABEX), TULIP (ANR-10-LABX-41 to N. Peeters and F. Maviane-Macia), and Colciencias Depar-tamento Administrativo de Ciencia, Tecnología e Innovació grant 673 (to C. Mazo-Molina). Publisher Copyright: © 2019 The American Phytopathological Society.

PY - 2019

Y1 - 2019

N2 - Race 1 strains of Pseudomonas syringae pv. tomato, which cause bacterial speck disease of tomato, are becoming increasingly common and no simply inherited genetic resistance to such strains is known. We discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 P. syringae pv. tomato strains by detecting the activity of type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades the RIN4 protein, thereby activating RPS2-mediated immunity. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 also detected the activity of AvrRpt2 homologs from diverse bacteria, including one in Ralstonia pseudosolanacearum. The genome sequence of S. lycopersicoides revealed no RPS2 homolog in the Ptr1 region. Ptr1 could play an important role in controlling bacterial speck disease and its future cloning may shed light on an example of convergent evolution for recognition of a widespread type III effector.

AB - Race 1 strains of Pseudomonas syringae pv. tomato, which cause bacterial speck disease of tomato, are becoming increasingly common and no simply inherited genetic resistance to such strains is known. We discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 P. syringae pv. tomato strains by detecting the activity of type III effector AvrRpt2. In Arabidopsis, AvrRpt2 degrades the RIN4 protein, thereby activating RPS2-mediated immunity. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 also detected the activity of AvrRpt2 homologs from diverse bacteria, including one in Ralstonia pseudosolanacearum. The genome sequence of S. lycopersicoides revealed no RPS2 homolog in the Ptr1 region. Ptr1 could play an important role in controlling bacterial speck disease and its future cloning may shed light on an example of convergent evolution for recognition of a widespread type III effector.

KW - AvrRpt2

KW - Disease resistance

KW - Mr5

KW - NLR-triggered immunity

KW - RIN4

KW - RPS2

KW - Solanum lycopersicoides

KW - Solanum lycopersicum

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U2 - 10.1094/MPMI-01-19-0018-R

DO - 10.1094/MPMI-01-19-0018-R

M3 - Article

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SN - 0894-0282

VL - 32

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JO - Molecular Plant-Microbe Interactions

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IS - 8

ER -

Mazo-Molina C, Mainiero S, Hind SR, Kraus CM, Vachev M, Maviane-Macia F et al. The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN. Molecular Plant-Microbe Interactions. 2019;32(8):946-960. doi: 10.1094/MPMI-01-19-0018-R

The ptr1 locus of solanum lycopersicoides confers resistance to race 1 strains of pseudomonas syringae pv. tomato and to ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2 and RipBN

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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