The protein chaperone Ssa1 affects mRNA localization to the mitochondria

Erez Eliyahu, Chen Lesnik, Yoav Arava

Research output: Contribution to journalArticlepeer-review


Many nuclear-transcribed mRNAs encoding mitochondrial proteins are localized near the mitochondrial outer membrane. A yet unresolved question is whether protein synthesis is important for transport of these mRNAs to their destination. Herein we present a connection between mRNA localization in yeast and the protein chaperone Ssa1. Ssa1 depletion lowered mRNA association with mitochondria while its overexpression increased it. A genome-wide analysis revealed that Ssa proteins preferentially affect mRNAs encoding hydrophobic proteins, which are expected targets for these protein chaperones. Importantly, deletion of the mitochondrial receptor Tom70 abolished the impact of Ssa1 overexpression on mRNAs encoding Tom70 targets. Taken together, our results suggest a role for Ssa1 in mediating localization of nascent peptide-ribosome-mRNA complexes to the mitochondria, consistent with a co-translational transport process.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalFEBS Letters
Issue number1
StatePublished - 2 Jan 2012
Externally publishedYes


  • Hsp70 chaperone
  • Mitochondria
  • Ssa1
  • Tom70
  • Yeast
  • mRNA localization

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


Dive into the research topics of 'The protein chaperone Ssa1 affects mRNA localization to the mitochondria'. Together they form a unique fingerprint.

Cite this