The primary structure of the hemoglobin of the mole rat (spalax ehrenbergi, rodentia, chromosome species 60)*

Traute Kleinschmidt, Eviatar Nevo, Gerhard Braunitzer

Research output: Contribution to journalArticlepeer-review

Abstract

Mole rat (Spalax ehrenbergi) hemoglobin consists of only one component. The complete amino-acid sequence of the α- and β-chains of the species with the diploid chromosome number of 60 is presented. Following chain separation by chromatography on carboxy-methyl cellulose CM-52, the primary structures were established by automatic Edman degradation on the chains, on the tryptic peptides, and on a peptide obtained by acid hydrolysis of the Asp-Pro bond in β-chains. The alignment of the peptides was performed by homology with human α- and β-chains. The comparison showed an exchange of 23 residues in the α-chains and 26 in the β-chains. One substitution in the β-chains concerns the surrounding of the heme. We found two exchanges in each chain in the α1βl -subunit interface and one in the ß-chain α1β2-contact points. Though all binding sites for 2,3-bisphosphogly-cerate are unchanged, the mole rat blood has a high oxygen affinity as a part of adaptation to subterranean life under hypoxia and hyper-capnia. A comparison of the sequence with known X-ray models of hemoglobins may give an interpretation of this fact. The primary structure of the mole rat hemoglobin shows more similarities with surface rodents, than with the mole, another small mammal, adapted to hypoxia in subterranean tunnels. The adaptation to hypoxia in mole rat and mole must be due to different mechanisms.

Original languageEnglish
Pages (from-to)531-538
Number of pages8
JournalHoppe-Seyler's Zeitschrift fur Physiologische Chemie
Volume365
Issue number1
DOIs
StatePublished - 1984
Externally publishedYes

Keywords

  • Mole rat
  • Rodentia
  • hemoglobin
  • primary structure

ASJC Scopus subject areas

  • Biochemistry

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