The genome sequence of the colonial chordate, Botryllus schlosseri

Ayelet Voskoboynik; Norma F. Neff; Debashis Sahoo; Aaron M. Newman; Dmitry Pushkarev; Winston Koh; Benedetto Passarelli; H. Christina Fan; Gary L. Mantalas; Karla J. Palmeri; Katherine J. Ishizuka; Carmela Gissi; Francesca Griggio; Rachel Ben-Shlomo; Daniel M. Corey; Lolita Penland; Richard A. White; Irving L. Weissman; Stephen R. Quake

doi:10.7554/eLife.00569

The genome sequence of the colonial chordate, Botryllus schlosseri

Ayelet Voskoboynik, Norma F. Neff, Debashis Sahoo, Aaron M. Newman, Dmitry Pushkarev, Winston Koh, Benedetto Passarelli, H. Christina Fan, Gary L. Mantalas, Karla J. Palmeri, Katherine J. Ishizuka, Carmela Gissi, Francesca Griggio, Rachel Ben-Shlomo, Daniel M. Corey, Lolita Penland, Richard A. White, Irving L. Weissman, Stephen R. Quake

Research output: Contribution to journal › Article › peer-review

Abstract

Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confdently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration.

Original language	English
Article number	e00569
Journal	eLife
Volume	2013
Issue number	2
DOIs	https://doi.org/10.7554/eLife.00569
State	Published - 2 Jul 2013

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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Voskoboynik, A., Neff, N. F., Sahoo, D., Newman, A. M., Pushkarev, D., Koh, W., Passarelli, B., Fan, H. C., Mantalas, G. L., Palmeri, K. J., Ishizuka, K. J., Gissi, C., Griggio, F., Ben-Shlomo, R., Corey, D. M., Penland, L., White, R. A., Weissman, I. L., & Quake, S. R. (2013). The genome sequence of the colonial chordate, Botryllus schlosseri. eLife, 2013(2), Article e00569. https://doi.org/10.7554/eLife.00569

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abstract = "Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confdently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration.",

author = "Ayelet Voskoboynik and Neff, {Norma F.} and Debashis Sahoo and Newman, {Aaron M.} and Dmitry Pushkarev and Winston Koh and Benedetto Passarelli and Fan, {H. Christina} and Mantalas, {Gary L.} and Palmeri, {Karla J.} and Ishizuka, {Katherine J.} and Carmela Gissi and Francesca Griggio and Rachel Ben-Shlomo and Corey, {Daniel M.} and Lolita Penland and White, {Richard A.} and Weissman, {Irving L.} and Quake, {Stephen R.}",

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Voskoboynik, A, Neff, NF, Sahoo, D, Newman, AM, Pushkarev, D, Koh, W, Passarelli, B, Fan, HC, Mantalas, GL, Palmeri, KJ, Ishizuka, KJ, Gissi, C, Griggio, F, Ben-Shlomo, R, Corey, DM, Penland, L, White, RA, Weissman, IL & Quake, SR 2013, 'The genome sequence of the colonial chordate, Botryllus schlosseri', eLife, vol. 2013, no. 2, e00569. https://doi.org/10.7554/eLife.00569

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AU - Griggio, Francesca

AU - Ben-Shlomo, Rachel

AU - Corey, Daniel M.

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AU - White, Richard A.

AU - Weissman, Irving L.

AU - Quake, Stephen R.

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The genome sequence of the colonial chordate, Botryllus schlosseri

Abstract

ASJC Scopus subject areas

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