The genome sequence of the colonial chordate, Botryllus schlosseri

Ayelet Voskoboynik, Norma F. Neff, Debashis Sahoo, Aaron M. Newman, Dmitry Pushkarev, Winston Koh, Benedetto Passarelli, H. Christina Fan, Gary L. Mantalas, Karla J. Palmeri, Katherine J. Ishizuka, Carmela Gissi, Francesca Griggio, Rachel Ben-Shlomo, Daniel M. Corey, Lolita Penland, Richard A. White, Irving L. Weissman, Stephen R. Quake

Research output: Contribution to journalArticlepeer-review


Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confdently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration.

Original languageEnglish
Article numbere00569
Issue number2
StatePublished - 2 Jul 2013

ASJC Scopus subject areas

  • Neuroscience (all)
  • Biochemistry, Genetics and Molecular Biology (all)
  • Immunology and Microbiology (all)


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