The evidence for a microRNA product of human DROSHA gene

Peter Mechtler, Sydney Johnson, Hannah Slabodkin, Amir B. Cohanim, Leonid Brodsky, Eugene S. Kandel

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNAs are short RNA molecules that regulate function and stability of a large subset of eukaryotic mRNAs. In the main pathway of microRNA biogenesis, a short “hairpin” is excised from a primary transcript by ribonuclease DROSHA, followed by additional nucleolytic processing by DICER and inclusion of the mature microRNA into the RNA-induced silencing complex. We report that a microRNA-like molecule is encoded by human DROSHA gene within a predicted stem-loop element of the respective transcript. This putative mature microRNA is complementary to DROSHA transcript variant 1 and can attenuate expression of the corresponding protein. The findings suggest a possibility for a negative feedback loop, wherein DROSHA processes its own transcript and produces an inhibitor of its own biosynthesis.

Original languageEnglish
Pages (from-to)1508-1513
Number of pages6
JournalRNA Biology
Volume14
Issue number11
DOIs
StatePublished - 2 Nov 2017

Bibliographical note

Funding Information:
We would like to thank Dr. Olejniczak for sharing reagents. The work was supported in part by the Roswell Park Alliance Foundation. The use of Roswell Park Cancer Institute’s shared resources is supported through the National Cancer Institute (NCI) grant P30CA016056. The participation of HS and SJ was made possible through Science Research Program at the Buffalo City Honors School.

Funding Information:
We would like to thank Dr. Olejniczak for sharing reagents. The work was supported in part by the Roswell Park Alliance Foundation. The use of Roswell Park Cancer Institute's shared resources is supported through the National Cancer Institute (NCI) grant P30CA016056. The participation of HS and SJ was made possible through Science Research Program at the Buffalo City Honors School.

Publisher Copyright:
© 2017 Taylor & Francis Group, LLC.

Keywords

  • DROSHA
  • RNA interference
  • microRNA biogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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