The evidence for a microRNA product of human DROSHA gene

Peter Mechtler; Sydney Johnson; Hannah Slabodkin; Amir B. Cohanim; Leonid Brodsky; Eugene S. Kandel

doi:10.1080/15476286.2017.1342934

The evidence for a microRNA product of human DROSHA gene

Peter Mechtler, Sydney Johnson, Hannah Slabodkin, Amir B. Cohanim, Leonid Brodsky, Eugene S. Kandel

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

MicroRNAs are short RNA molecules that regulate function and stability of a large subset of eukaryotic mRNAs. In the main pathway of microRNA biogenesis, a short “hairpin” is excised from a primary transcript by ribonuclease DROSHA, followed by additional nucleolytic processing by DICER and inclusion of the mature microRNA into the RNA-induced silencing complex. We report that a microRNA-like molecule is encoded by human DROSHA gene within a predicted stem-loop element of the respective transcript. This putative mature microRNA is complementary to DROSHA transcript variant 1 and can attenuate expression of the corresponding protein. The findings suggest a possibility for a negative feedback loop, wherein DROSHA processes its own transcript and produces an inhibitor of its own biosynthesis.

Original language	English
Pages (from-to)	1508-1513
Number of pages	6
Journal	RNA Biology
Volume	14
Issue number	11
DOIs	https://doi.org/10.1080/15476286.2017.1342934
State	Published - 2 Nov 2017

Bibliographical note

Funding Information:
We would like to thank Dr. Olejniczak for sharing reagents. The work was supported in part by the Roswell Park Alliance Foundation. The use of Roswell Park Cancer Institute’s shared resources is supported through the National Cancer Institute (NCI) grant P30CA016056. The participation of HS and SJ was made possible through Science Research Program at the Buffalo City Honors School.

Funding Information:
We would like to thank Dr. Olejniczak for sharing reagents. The work was supported in part by the Roswell Park Alliance Foundation. The use of Roswell Park Cancer Institute's shared resources is supported through the National Cancer Institute (NCI) grant P30CA016056. The participation of HS and SJ was made possible through Science Research Program at the Buffalo City Honors School.

Publisher Copyright:
© 2017 Taylor & Francis Group, LLC.

Keywords

DROSHA
RNA interference
microRNA biogenesis

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1080/15476286.2017.1342934

Cite this

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abstract = "MicroRNAs are short RNA molecules that regulate function and stability of a large subset of eukaryotic mRNAs. In the main pathway of microRNA biogenesis, a short “hairpin” is excised from a primary transcript by ribonuclease DROSHA, followed by additional nucleolytic processing by DICER and inclusion of the mature microRNA into the RNA-induced silencing complex. We report that a microRNA-like molecule is encoded by human DROSHA gene within a predicted stem-loop element of the respective transcript. This putative mature microRNA is complementary to DROSHA transcript variant 1 and can attenuate expression of the corresponding protein. The findings suggest a possibility for a negative feedback loop, wherein DROSHA processes its own transcript and produces an inhibitor of its own biosynthesis.",

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author = "Peter Mechtler and Sydney Johnson and Hannah Slabodkin and Cohanim, {Amir B.} and Leonid Brodsky and Kandel, {Eugene S.}",

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The evidence for a microRNA product of human DROSHA gene

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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