The effect of α- and β-adrenergic blockade on daily rhythms of body temperature, urine production, and urinary 6-sulfatoxymelatonin of social voles Microtus socialis

To examine the effect of adrenergic blockade on daily rhythms of rectal body temperature (T_b), urine production rate, and melatonin (MEL; measured as urinary 6-sulfatoxymelatonin; 6-SMT), social voles Microtus socialis received a single intra-peritoneal injection of either prazosin (PRAZ, 1 mg/kg) or propranolol (PROP, 4.5 mg/kg); α- and β-adrenergic blocking agents respectively, 1 h prior to scotophase onset (light/dark, 12L:12D; lights on 07:00 h). Both blockers caused significant decrease in T_b values mainly during scotophase. Nocturnal urine production rates were higher for M. socialis treated with the drugs compared with controls. Overall, urine production rates were systematically higher in PROP-voles over the 24 h period when compared with PRAZ-voles; however these differences were not statistically significant. Interestingly, PROP caused significant elevation in urinary 6-SMT at the second half of the dark phase, whereas PRAZ had no effects. These data suggest that the mechanisms regulating MEL synthesis and thermoregulatory responses in M. socialis are different from those described in other rodents' species. Importantly, the data also suggest that the β-blockade-induced elevation in MEL levels may be directly associated with increased urination in M. socialis.