The differential role of cortical protein synthesis in taste memory formation and persistence

David Levitan, Shunit Gal-Ben-ari, Christopher Heise, Tali Rosenberg, Alina Elkobi, Sharon Inberg, Carlo Sala, Kobi Rosenblum

Research output: Contribution to journalArticlepeer-review

Abstract

The current dogma suggests that the formation of long-term memory (LTM) is dependent on protein synthesis but persistence of the memory trace is not. However, many of the studies examining the effect of protein synthesis inhibitors (PSIs) on LTM persistence were performed in the hippocampus, which is known to have a time-dependent role in memory storage, rather than the cortex, which is considered to be the main structure to store long-term memories. Here we studied the effect of PSIs on LTM formation and persistence in male Wistar Hola (n≥5) rats by infusing the protein synthesis inhibitor, anisomycin (100 μg, 1 μl), into the gustatory cortex (GC) during LTM formation and persistence in conditioned taste aversion (CTA). We found that local anisomycin infusion to the GC before memory acquisition impaired LTM formation (P = 8.9E − 5), but had no effect on LTM persistence when infused 3 days post acquisition (P = 0.94). However, when we extended the time interval between treatment with anisomycin and testing from 3 days to 14 days, LTM persistence was enhanced (P = 0.01). The enhancement was on the background of stable and non-declining memory, and was not recapitulated by another amnesic agent, APV (10 μg, 1 μl), an N-methyl-D-aspartate receptor antagonist (P = 0.54). In conclusion, CTA LTM remains sensitive to the action of PSIs in the GC even 3 days following memory acquisition. This sensitivity is differentially expressed between the formation and persistence of LTM, suggesting that increased cortical protein synthesis promotes LTM formation, whereas decreased protein synthesis promotes LTM persistence.

Original languageEnglish
Article number16001
Journalnpj Science of Learning
Volume1
DOIs
StatePublished - 2016

Bibliographical note

Funding Information:
This work was supported by European Union Seventh Framework Program EUROSPIN (Contract HEALTH-F2-2009-241498) and the German-Israeli Foundation DIP (RO3971/1-1) grants to K.R.; by Comitato Telethon Fondazione Onlus, grant GGP13187, Fondazione CARIPLO project number 2012.0593 and Regione Lobardia, Nutec project to C.S. C.H. was supported by SyMBaD (ITN MarieCurie, Grant Agreement no. 238608—7th Framework Programme of the EU) to C.S.

Publisher Copyright:
© The Author(s) 2016.

ASJC Scopus subject areas

  • Education
  • Developmental Neuroscience

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