Abstract
This research compares the performance and diagnostic accuracy of three molecular tests for the detection of Clostridium difficile in stool samples, with the Xpert® C. difficile assay. Fifty-nine positive and twenty-five stool negative samples were analyzed by the BDmax™ Cdiff, the Simplexa C. difficile Direct, and the GenomEra™ C. difficile, and compared with the Xpert® C. difficile routinely used in our laboratory. The highest sensitivity was 94.9% for the BDmax™ Cdiff, followed by GenomEra™ C. difficile with 93.2%, and Simplexa™ C. difficile Direct with 89.8%. The specificities of all assays were 100%. GenomEra™ C. difficile had the highest retesting rate (12%). Simplexa™ C. difficile Direct benefits from both short hands-on time and total-turnaround time and 0% retesting. The differences in performance and accuracy between these three molecular assays are insignificant and all may be used as part of the routine algorithm for detection of C. difficile.
Original language | English |
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Article number | 105784 |
Journal | Journal of Microbiological Methods |
Volume | 168 |
DOIs | |
State | Published - Jan 2020 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2019 Elsevier B.V.
Keywords
- Clostridium difficile
- Diagnostic accuracy
- Molecular assays
- Performance
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Microbiology (medical)