Temporal and spatial expression patterns of biomineralization proteins during early development in the stony coral Pocillopora damicornis

Tali Mass, Hollie M. Putnam, Jeana L. Drake, Ehud Zelzion, Ruth D. Gates, Debashish Bhattacharya, Paul G. Falkowski

Research output: Contribution to journalArticlepeer-review

Abstract

Reef-building corals begin as non-calcifying larvae that, upon settling, rapidly begin to accrete skeleton and a protein-rich skeletal organic matrix that attach them to the reef. Here, we characterized the temporal and spatial expression pattern of a suite of biomineralization genes during three stages of larval development in the reef-building coral Pocillopora damicornis: stage I, newly released; stage II, oral-aborally compressed and stage III, settled and calcifying spat. Transcriptome analysis revealed 3882 differentially expressed genes that clustered into four distinctly different patterns of expression change across the three developmental stages. Immunolocalization analysis further reveals the spatial arrangement of coral acid-rich proteins (CARPs) in the overall architecture of the emerging skeleton. These results provide the first analysis of the timing of the biomineralization ‘toolkit’ in the early life history of a stony coral.

Original languageEnglish
Article number20160322
JournalProceedings of the Royal Society B: Biological Sciences
Volume283
Issue number1829
DOIs
StatePublished - 27 Apr 2016

Bibliographical note

Funding Information:
This work was made possible by a grant from the National Science Foundation (EF-1416785) awarded to P.G.F., D.B. and T.M. and to H.M.P. (OCE-PRF-1323822).

Publisher Copyright:
© 2016 The Author(s) Published by the Royal Society. All rights reserved.

Keywords

  • Acidic proteins
  • Biomineralization
  • Coral development
  • Gene expression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Immunology and Microbiology (all)
  • Environmental Science (all)
  • Agricultural and Biological Sciences (all)

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