TdCBL6, a calcineurin B-like gene from wild emmer wheat (Triticum dicoccoides), is involved in response to salt and low-K+ stresses

Liang Chen, Jing Ren, Haiyan Shi, Yongkuan Zhang, Yu You, Jibiao Fan, Ke Chen, Shuqian Liu, Eviatar Nevo, Jinming Fu, Junhua Peng

Research output: Contribution to journalArticlepeer-review

Abstract

The calcineurin B-like proteins (CBLs), a unique family of calcium sensors in plants, have been shown to be involved in abiotic stresses, such as salt, drought and cold. Although extensive studies and remarkable progress have been made in Arabidopsis (Arabidopsis thaliana) CBLs, very little is known about the role of CBL genes in wheat. In this study, a CBL gene, designated TdCBL6, was cloned and characterized from wild emmer wheat (Triticum dicoccoides), the progenitor of cultivated wheat. Sequence alignment revealed that TdCBL6 shares high sequence homology with rice OsCBL6. Phylogenetic analysis also revealed that TdCBL6 protein has the closest evolutionary relationship with rice OsCBL6 protein. TdCBL6 transcription was induced by NaCl, polyethylene glycol and abscisic acid. Further differential expression analysis revealed that TdCBL6 expression was much higher in the salt-tolerant line than in the salt-sensitive line when they were subjected to salt treatment. Transgenic Arabidopsis ectopic expression of the TdCBL6 gene displayed higher levels of photosynthetic efficiency (Fv/Fm) and lower ion leakage (EL) than wild-type (WT) plants under NaCl stress conditions. Moreover, TdCBL6-overexpressing lines showed low-K+ (LK)-sensitive phenotypes compared with WT plants. Further experiments revealed that ectopic expression of TdCBL6 resulted in reduction of H2O2 content, and affected expression of K+-responsive/H2O2-regulated genes under LK stress. Taken together, we demonstrated that heterologous expression of TdCBL6 in Arabidopsis confers salt tolerance by reducing membrane injury and improving photosynthetic efficiency, and that the TdCBL6 gene may be involved in response to LK stress by regulating the reactive oxygen species-mediated LK signaling pathway.

Original languageEnglish
JournalMolecular Breeding
Volume35
Issue number1
DOIs
StatePublished - Jan 2015

Bibliographical note

Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.

Keywords

  • Abiotic stress
  • CBL protein
  • Low-K
  • Salt tolerance
  • Wild emmer wheat

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science
  • Biotechnology
  • Plant Science

Fingerprint

Dive into the research topics of 'TdCBL6, a calcineurin B-like gene from wild emmer wheat (Triticum dicoccoides), is involved in response to salt and low-K+ stresses'. Together they form a unique fingerprint.

Cite this