Substances from the medicinal mushroom Daedalea gibbosa inhibit kinase activity of native and T315I mutated Bcr-Abl

Majed Yassin, Solomon P. Wasser, Jamal Mahajna

Research output: Contribution to journalArticlepeer-review


Human chronic myelogenous leukemia (CML) is a malignancy of pluripotent hematopoietic cells characterized by a distinctive cytogenetic abnormality resulting in the creation of a p210 Bcr-Abl fusion protein with abnormal tyrosine kinase activity. Recently, a selective Abl kinase inhibitor, Imatinib mesylate, was introduced as a first line therapy for CML. Despite the initial response, CML patients develop a resistantance to Imatinib, which is mediated mainly by point mutations within the Ab1 protein. Herein, we describe the identification of mycelium organic extracts of Daedalea gibbosa with selective anti-proliferating and apoptosis-inducing activities against K562 cells and other laboratory model of CML. Using activity-guided purification, we isolated an active fraction, F6, which inhibits in vitro kinase activity of recombinant Ab1. The active fraction significantly inhibits the autophosphorylation of native and mutated Bcr-Ab1, which are resistant to Imatinib treatment including the T315I mutation. Using a colony-forming assay, we demonstrated that the active fraction is effective in inhibiting the colony formation of the Ba/F3 cell line harboring either native Bcr-Ab1 or its mutations, including the T315I mutation. Our data illustrated the potential of natural products in cancer therapeutics.

Original languageEnglish
Pages (from-to)1197-1204
Number of pages8
JournalInternational Journal of Oncology
Issue number6
StatePublished - Jun 2008


  • Antitumor activity
  • Apoptosis
  • Bcr-Abl
  • Chronic myelogenous leukemia
  • Daedalea gibbosa
  • Differentiation
  • Kinase
  • Medicinal mushrooms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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