Striving for normality: Whole body regeneration through a series of abnormal generations

Ayelet Voskoboynik, Noa Simon-Blecher, Yoav Soen, Baruch Rinkevich, Anthony W. De Tomaso, Katherine J. Ishizuka, Irving L. Weissman

Research output: Contribution to journalArticlepeer-review

Abstract

Embryogenesis and asexual reproduction are commonly considered to be coordinated developmental processes, which depend on accurate progression through a defined sequence of developmental stages. Here we report a peculiar developmental scenario in a simple chordate, Botryllus schlosseri, wherein a normal colony of individuals (zooids and buds) is regenerated from the vasculature (vascular budding) through a sequence of morphologically abnormal developmental stages. Vascular budding was induced by surgically removing buds and zooids from B. schlosseri colonies, leaving only the vasculature and the tunic that connects them. In vivo imaging and histological sections showed that the timing and morphology of developing structures during vascular budding deviated signifi-cantly from other asexual reproduction modes (the regular asexual reproduction mode in this organism and vascular budding in other botryllid species). Subsequent asexual reproduction cycles exhibited gradual regaining of normal developmental patterns, eventually leading to regeneration of a normal colony. The conversion into a normal body form suggests the activation of an alternative pathway of asexual reproduction, which involves gradual regaining of normal positional information. It presents a powerful model for studying the specification of the same body plan by different developmental programs.

Original languageEnglish
Pages (from-to)1335-1344
Number of pages10
JournalFASEB Journal
Volume21
Issue number7
DOIs
StatePublished - May 2007
Externally publishedYes

Keywords

  • Blastogenesis
  • Development
  • Tunicate
  • Vascular budding

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Biotechnology

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