Glycosides are a major group of plant secondary compounds characterized by one or more sugars conjugated to a lipophilic, possibly toxic aglycone, which is released upon hydrolysis. We compared small intestinal homogenate hydrolysis activity of three rodent and two avian species against four substrates: amygdalin and sinigrin, two plant-derived glucosides, the sugar lactose, whose hydrolysis models some activity against flavonoid and isoflavonoid glucosides, and the disaccharide sugar maltose (from starch), used as a comparator. Three new findings extend our understanding of physiological processing of plant glucosides: (1) the capacity of passerine birds to hydrolyze plant glucosides seems relatively low, compared with rodents; (2) in this first test of vertebrates' enzymic capacity to hydrolyze glucosinolates, sinigrin hydrolytic capacity seems low; (3) in laboratory mice, hydrolytic activity against lactose resides on the enterocytes' apical membrane facing the intestinal lumen, but activity against amygdalin seems to reside inside enterocytes.
|Number of pages||4|
|Journal||Journal of Experimental Biology|
|State||Published - 1 Sep 2015|
Bibliographical notePublisher Copyright:
© 2015. Published by The Company of Biologists Ltd.
- Cytosolic β-glucosidase
- Lactase phlorizin hydrolase
- Plant secondary compounds
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Aquatic Science
- Animal Science and Zoology
- Molecular Biology
- Insect Science