Short-and long-term effects of juvenile stressor exposure on the expression of GABA

Shlomit Jacobson-Pick, Gal Richter-Levin

Research output: Contribution to journalArticlepeer-review

Abstract

During the juvenile period rodents are particularly sensitive to stressors. Aversive events encountered during this period may have enduring effects that are not evident among animals initially stressed as adults. Interestingly, experiencing stressor during juvenile period was found to elicit a biphasic behavioral pattern over the course of development. During the juvenile period, the expression of several GABAA receptor subunits is subject to elevated plasticity, rendering the GABAergic system sensitive to stressors. In the present investigation, animals were exposed to a juvenile variable stressor regimen (JUV-S) at 2729 postnatal days (PND): 27 PNDacute swim stress (10 min), 28 PNDelevated platform stress (3 sessions × 30 min each), and 29 PNDrestraint (2 h). One hour following the last exposure to stressor or in adulthood (60 PND), anxiety-related behaviors were assessed in a 5-min elevated plus maze test. The western blotting technique was used to evaluate whether the juvenile stress induced behavioral pattern will be accompanied by respective changes in GABAA α1, α2, and α3 protein expression in male rats. Our findings further established that juvenile stressor elicits hyper-reactivity when rats were tested as juveniles, whereas rats exhibited reduced activity and increased anxiety when tested as adults. Additionally, the effects of juvenile stressor on α1, α2, and α3 were more pronounced among juvenile stressed rats that were challenged as adults compared with rats that were only challenged as juveniles. Interestingly, the stress-induced modulation of the subunits was particularly evident in the amygdala, a brain region closely associated with anxiety. Thus, age-and region-specific alterations of the α subunits may contribute to the age-specific behavioral alterations observed following juvenile stress exposure.

Original languageEnglish
Pages (from-to)416-424
Number of pages9
JournalStress
Volume15
Issue number4
DOIs
StatePublished - Jul 2012

Bibliographical note

Funding Information:
Declaration of interest: This work was funded by a Deutsche Israel Project cooperation grant to G.R-L and by a grant from the Institute for the Study of Affective Neuroscience, University of Haifa, which was endowed by the Hope for Depression Research Foundation, to G.R-L. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Keywords

  • Amygdala
  • Anxiety
  • Elevated plus maze
  • GABAA receptor
  • Hippocampus
  • Juvenile stressor

ASJC Scopus subject areas

  • Endocrine and Autonomic Systems
  • Neuropsychology and Physiological Psychology
  • Psychiatry and Mental health
  • Physiology
  • Behavioral Neuroscience

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