Shoot regeneration from leaf explants of Brunfelsia calycina

Raya Liberman, Liat Shahar, Ada Nissim-Levi, Dalia Evenor, Moshe Reuveni, Michal Oren-Shamir

Research output: Contribution to journalArticlepeer-review


A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23-2.46 μM indole butrytic acid (IBA), they developed roots.

Original languageEnglish
Pages (from-to)345-348
Number of pages4
JournalPlant Cell, Tissue and Organ Culture
Issue number3
StatePublished - Jan 2010
Externally publishedYes


  • Brunfelsia
  • Regeneration
  • Solanaceae

ASJC Scopus subject areas

  • Horticulture


Dive into the research topics of 'Shoot regeneration from leaf explants of Brunfelsia calycina'. Together they form a unique fingerprint.

Cite this