Shoot regeneration from leaf explants of Brunfelsia calycina

Raya Liberman; Liat Shahar; Ada Nissim-Levi; Dalia Evenor; Moshe Reuveni; Michal Oren-Shamir

doi:10.1007/s11240-009-9642-7

Shoot regeneration from leaf explants of Brunfelsia calycina

Raya Liberman, Liat Shahar, Ada Nissim-Levi, Dalia Evenor, Moshe Reuveni, Michal Oren-Shamir

Research output: Contribution to journal › Article › peer-review

Abstract

A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23-2.46 μM indole butrytic acid (IBA), they developed roots.

Original language	English
Pages (from-to)	345-348
Number of pages	4
Journal	Plant Cell, Tissue and Organ Culture
Volume	100
Issue number	3
DOIs	https://doi.org/10.1007/s11240-009-9642-7
State	Published - Jan 2010
Externally published	Yes

Keywords

Brunfelsia
Regeneration
Solanaceae

ASJC Scopus subject areas

Horticulture

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10.1007/s11240-009-9642-7

Cite this

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title = "Shoot regeneration from leaf explants of Brunfelsia calycina",

abstract = "A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23-2.46 μM indole butrytic acid (IBA), they developed roots.",

keywords = "Brunfelsia, Regeneration, Solanaceae",

author = "Raya Liberman and Liat Shahar and Ada Nissim-Levi and Dalia Evenor and Moshe Reuveni and Michal Oren-Shamir",

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doi = "10.1007/s11240-009-9642-7",

language = "English",

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T1 - Shoot regeneration from leaf explants of Brunfelsia calycina

AU - Liberman, Raya

AU - Shahar, Liat

AU - Nissim-Levi, Ada

AU - Evenor, Dalia

AU - Reuveni, Moshe

AU - Oren-Shamir, Michal

PY - 2010/1

Y1 - 2010/1

N2 - A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23-2.46 μM indole butrytic acid (IBA), they developed roots.

AB - A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23-2.46 μM indole butrytic acid (IBA), they developed roots.

KW - Brunfelsia

KW - Regeneration

KW - Solanaceae

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ER -

Shoot regeneration from leaf explants of Brunfelsia calycina

Abstract

Keywords

ASJC Scopus subject areas

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