Sequential estimation of linkage between PCR-generated markers and a target gene employing stepwise bulked analysis

Yefim I. Ronin; Abraham B. Korol; Tzion Fahima; Valery M. Kirzhner; Eviatar Nevo

doi:10.2307/2532856

Sequential estimation of linkage between PCR-generated markers and a target gene employing stepwise bulked analysis

Yefim I. Ronin, Abraham B. Korol, Tzion Fahima, Valery M. Kirzhner, Eviatar Nevo

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

PCR-based markers are used for targeting plant genes. However, mapping these markers requires laborious and expensive analysis of individual genotypes. We propose here a sequential procedure for fine mapping of PCR markers relative to a target gene. Stepwise bulked analysis is employed to get a censored estimation of the recombination rate. The sequential estimation is compared to fixed sample size design. In both cases, the proposed procedure can achieve a substantial reduction in the number of PCR runs (up to 90 97% for close linkage) as compared to the standard individual- by-individual analysis.

Original language	English
Pages (from-to)	1428-1439
Number of pages	12
Journal	Biometrics
Volume	52
Issue number	4
DOIs	https://doi.org/10.2307/2532856
State	Published - Dec 1996

Keywords

Bias
Bulked analysis
Censored estimation
Close linkage
Multiple markers
PCR
Sequential analysis

ASJC Scopus subject areas

Statistics and Probability
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Agricultural and Biological Sciences
Applied Mathematics

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10.2307/2532856

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title = "Sequential estimation of linkage between PCR-generated markers and a target gene employing stepwise bulked analysis",

abstract = "PCR-based markers are used for targeting plant genes. However, mapping these markers requires laborious and expensive analysis of individual genotypes. We propose here a sequential procedure for fine mapping of PCR markers relative to a target gene. Stepwise bulked analysis is employed to get a censored estimation of the recombination rate. The sequential estimation is compared to fixed sample size design. In both cases, the proposed procedure can achieve a substantial reduction in the number of PCR runs (up to 90 97\% for close linkage) as compared to the standard individual- by-individual analysis.",

keywords = "Bias, Bulked analysis, Censored estimation, Close linkage, Multiple markers, PCR, Sequential analysis",

author = "Ronin, \{Yefim I.\} and Korol, \{Abraham B.\} and Tzion Fahima and Kirzhner, \{Valery M.\} and Eviatar Nevo",

year = "1996",

month = dec,

doi = "10.2307/2532856",

language = "English",

volume = "52",

pages = "1428--1439",

journal = "Biometrics",

issn = "0006-341X",

publisher = "Oxford University Press",

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T1 - Sequential estimation of linkage between PCR-generated markers and a target gene employing stepwise bulked analysis

AU - Ronin, Yefim I.

AU - Korol, Abraham B.

AU - Fahima, Tzion

AU - Kirzhner, Valery M.

AU - Nevo, Eviatar

PY - 1996/12

Y1 - 1996/12

N2 - PCR-based markers are used for targeting plant genes. However, mapping these markers requires laborious and expensive analysis of individual genotypes. We propose here a sequential procedure for fine mapping of PCR markers relative to a target gene. Stepwise bulked analysis is employed to get a censored estimation of the recombination rate. The sequential estimation is compared to fixed sample size design. In both cases, the proposed procedure can achieve a substantial reduction in the number of PCR runs (up to 90 97% for close linkage) as compared to the standard individual- by-individual analysis.

AB - PCR-based markers are used for targeting plant genes. However, mapping these markers requires laborious and expensive analysis of individual genotypes. We propose here a sequential procedure for fine mapping of PCR markers relative to a target gene. Stepwise bulked analysis is employed to get a censored estimation of the recombination rate. The sequential estimation is compared to fixed sample size design. In both cases, the proposed procedure can achieve a substantial reduction in the number of PCR runs (up to 90 97% for close linkage) as compared to the standard individual- by-individual analysis.

KW - Bias

KW - Bulked analysis

KW - Censored estimation

KW - Close linkage

KW - Multiple markers

KW - PCR

KW - Sequential analysis

UR - https://www.scopus.com/pages/publications/0030448973

U2 - 10.2307/2532856

DO - 10.2307/2532856

M3 - Article

C2 - 8962461

AN - SCOPUS:0030448973

SN - 0006-341X

VL - 52

SP - 1428

EP - 1439

JO - Biometrics

JF - Biometrics

IS - 4

ER -

Sequential estimation of linkage between PCR-generated markers and a target gene employing stepwise bulked analysis

Abstract

Keywords

ASJC Scopus subject areas

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