Sequence heterogeneity of nef transcripts in HIV-1-infected subjects at different stages of disease

Lee Ratner; Theresa Joseph; Juan Bandres; Sajal Ghosh; Nancy Vander Heyden; Alan Templeton; Beatrice Hahn; William Powderly; Max Arens

doi:10.1006/viro.1996.0474

Sequence heterogeneity of nef transcripts in HIV-1-infected subjects at different stages of disease

Lee Ratner, Theresa Joseph, Juan Bandres, Sajal Ghosh, Nancy Vander Heyden, Alan Templeton, Beatrice Hahn, William Powderly, Max Arens

Research output: Contribution to journal › Article › peer-review

Abstract

Nef transcripts were analyzed from peripheral blood mononuclear cells of 10 HIV-1-infected subjects with 9-822 CD4+ lymphocytes/cu mm, including 4 individuals with a probable common source infection. There was no relationship between the phylogenetic position of the various net sequences and the disease state of the person from whom they were derived. The nef open reading frame was disrupted in all three clones from only 1 subject. Functional analyses of a representative clone from each of the remaining 9 subjects showed that all nef alleles were capable of CD4 cell surface down-regulation, but only three naf alleles suppressed the induction of IL-2 transcription.

Original language	English
Pages (from-to)	245-250
Number of pages	6
Journal	Virology
Volume	223
Issue number	1
DOIs	https://doi.org/10.1006/viro.1996.0474
State	Published - 1 Sep 1996
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by PHS Grants AI24745, 25903 and grants from AMFAR and the McDonnell Douglas Employees Community Fund.

ASJC Scopus subject areas

Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/viro.1996.0474

Cite this

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title = "Sequence heterogeneity of nef transcripts in HIV-1-infected subjects at different stages of disease",

abstract = "Nef transcripts were analyzed from peripheral blood mononuclear cells of 10 HIV-1-infected subjects with 9-822 CD4+ lymphocytes/cu mm, including 4 individuals with a probable common source infection. There was no relationship between the phylogenetic position of the various net sequences and the disease state of the person from whom they were derived. The nef open reading frame was disrupted in all three clones from only 1 subject. Functional analyses of a representative clone from each of the remaining 9 subjects showed that all nef alleles were capable of CD4 cell surface down-regulation, but only three naf alleles suppressed the induction of IL-2 transcription.",

author = "Lee Ratner and Theresa Joseph and Juan Bandres and Sajal Ghosh and {Vander Heyden}, Nancy and Alan Templeton and Beatrice Hahn and William Powderly and Max Arens",

note = "Funding Information: This work was supported by PHS Grants AI24745, 25903 and grants from AMFAR and the McDonnell Douglas Employees Community Fund.",

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AU - Ratner, Lee

AU - Joseph, Theresa

AU - Bandres, Juan

AU - Ghosh, Sajal

AU - Vander Heyden, Nancy

AU - Templeton, Alan

AU - Hahn, Beatrice

AU - Powderly, William

AU - Arens, Max

N1 - Funding Information: This work was supported by PHS Grants AI24745, 25903 and grants from AMFAR and the McDonnell Douglas Employees Community Fund.

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Y1 - 1996/9/1

N2 - Nef transcripts were analyzed from peripheral blood mononuclear cells of 10 HIV-1-infected subjects with 9-822 CD4+ lymphocytes/cu mm, including 4 individuals with a probable common source infection. There was no relationship between the phylogenetic position of the various net sequences and the disease state of the person from whom they were derived. The nef open reading frame was disrupted in all three clones from only 1 subject. Functional analyses of a representative clone from each of the remaining 9 subjects showed that all nef alleles were capable of CD4 cell surface down-regulation, but only three naf alleles suppressed the induction of IL-2 transcription.

AB - Nef transcripts were analyzed from peripheral blood mononuclear cells of 10 HIV-1-infected subjects with 9-822 CD4+ lymphocytes/cu mm, including 4 individuals with a probable common source infection. There was no relationship between the phylogenetic position of the various net sequences and the disease state of the person from whom they were derived. The nef open reading frame was disrupted in all three clones from only 1 subject. Functional analyses of a representative clone from each of the remaining 9 subjects showed that all nef alleles were capable of CD4 cell surface down-regulation, but only three naf alleles suppressed the induction of IL-2 transcription.

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Sequence heterogeneity of nef transcripts in HIV-1-infected subjects at different stages of disease

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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