Separation of cytochrome P-450 containing vesicles from the midgut microsomal fraction of Manduca sexta

J. J. Neal; M. Reuveni

doi:10.1016/0742-8413(92)90047-B

Separation of cytochrome P-450 containing vesicles from the midgut microsomal fraction of Manduca sexta

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Abstract

1. Microsomes prepared from midgut tissue of Manduca sexta by differential centrifugation are a heterogeneous population of vesicles. 2. Upon centrifugation of microsomes in a sucrose gradient of 30%, 15% and 10%, specific activity of cytochrome P-450 catalyzed O-demethylation of p-nitroanisole (a marker enzyme for endoplasmic reticulum) was increased 2.5 to 3.5-fold in the 15% fraction. 3. Specific activities of alkaline phosphatase and leucine aminopeptidase (marker enzymes for brush border membranes) were increased 3.1 to 5.7-fold in the pellet. 4. Differential centrifugation is a useful step in the purification of cytochrome P-450 enzymes.

Original language	English
Pages (from-to)	77-82
Number of pages	6
Journal	Comparative biochemistry and physiology. C: Comparative pharmacology
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/0742-8413(92)90047-B
State	Published - May 1992
Externally published	Yes

ASJC Scopus subject areas

Immunology
Pharmacology

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title = "Separation of cytochrome P-450 containing vesicles from the midgut microsomal fraction of Manduca sexta",

abstract = "1. Microsomes prepared from midgut tissue of Manduca sexta by differential centrifugation are a heterogeneous population of vesicles. 2. Upon centrifugation of microsomes in a sucrose gradient of 30%, 15% and 10%, specific activity of cytochrome P-450 catalyzed O-demethylation of p-nitroanisole (a marker enzyme for endoplasmic reticulum) was increased 2.5 to 3.5-fold in the 15% fraction. 3. Specific activities of alkaline phosphatase and leucine aminopeptidase (marker enzymes for brush border membranes) were increased 3.1 to 5.7-fold in the pellet. 4. Differential centrifugation is a useful step in the purification of cytochrome P-450 enzymes.",

author = "Neal, {J. J.} and M. Reuveni",

year = "1992",

month = may,

doi = "10.1016/0742-8413(92)90047-B",

language = "English",

volume = "102",

pages = "77--82",

journal = "Comparative biochemistry and physiology. C: Comparative pharmacology",

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AU - Neal, J. J.

AU - Reuveni, M.

PY - 1992/5

Y1 - 1992/5

N2 - 1. Microsomes prepared from midgut tissue of Manduca sexta by differential centrifugation are a heterogeneous population of vesicles. 2. Upon centrifugation of microsomes in a sucrose gradient of 30%, 15% and 10%, specific activity of cytochrome P-450 catalyzed O-demethylation of p-nitroanisole (a marker enzyme for endoplasmic reticulum) was increased 2.5 to 3.5-fold in the 15% fraction. 3. Specific activities of alkaline phosphatase and leucine aminopeptidase (marker enzymes for brush border membranes) were increased 3.1 to 5.7-fold in the pellet. 4. Differential centrifugation is a useful step in the purification of cytochrome P-450 enzymes.

AB - 1. Microsomes prepared from midgut tissue of Manduca sexta by differential centrifugation are a heterogeneous population of vesicles. 2. Upon centrifugation of microsomes in a sucrose gradient of 30%, 15% and 10%, specific activity of cytochrome P-450 catalyzed O-demethylation of p-nitroanisole (a marker enzyme for endoplasmic reticulum) was increased 2.5 to 3.5-fold in the 15% fraction. 3. Specific activities of alkaline phosphatase and leucine aminopeptidase (marker enzymes for brush border membranes) were increased 3.1 to 5.7-fold in the pellet. 4. Differential centrifugation is a useful step in the purification of cytochrome P-450 enzymes.

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SN - 0306-4492

VL - 102

SP - 77

EP - 82

JO - Comparative biochemistry and physiology. C: Comparative pharmacology

JF - Comparative biochemistry and physiology. C: Comparative pharmacology

IS - 1

ER -

Separation of cytochrome P-450 containing vesicles from the midgut microsomal fraction of Manduca sexta

Abstract

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