Separable Roles for a Caenorhabditis elegans RMI1 Homolog in Promoting and Antagonizing Meiotic Crossovers Ensure Faithful Chromosome Inheritance

Marlène Jagut, Patricia Hamminger, Alexander Woglar, Sophia Millonigg, Luis Paulin, Martin Mikl, Maria Rosaria Dello Stritto, Lois Tang, Cornelia Habacher, Angela Tam, Miguel Gallach, Arndt von Haeseler, Anne M. Villeneuve, Verena Jantsch

Research output: Contribution to journalArticlepeer-review

Abstract

During the first meiotic division, crossovers (COs) between homologous chromosomes ensure their correct segregation. COs are produced by homologous recombination (HR)-mediated repair of programmed DNA double strand breaks (DSBs). As more DSBs are induced than COs, mechanisms are required to establish a regulated number of COs and to repair remaining intermediates as non-crossovers (NCOs). We show that the Caenorhabditis elegans RMI1 homolog-1 (RMH-1) functions during meiosis to promote both CO and NCO HR at appropriate chromosomal sites. RMH-1 accumulates at CO sites, dependent on known pro-CO factors, and acts to promote CO designation and enforce the CO outcome of HR-intermediate resolution. RMH-1 also localizes at NCO sites and functions in parallel with SMC-5 to antagonize excess HR-based connections between chromosomes. Moreover, RMH-1 also has a major role in channeling DSBs into an NCO HR outcome near the centers of chromosomes, thereby ensuring that COs form predominantly at off-center positions.

Original languageEnglish
Article numbere1002412
JournalPLoS Biology
Volume14
Issue number3
DOIs
StatePublished - 24 Mar 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 Jagut et al.

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Neuroscience

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