TY - JOUR
T1 - Sensitivity of primary fibroblasts in culture to atmospheric oxygen does not correlate with species lifespan
AU - Patrick, Alison
AU - Seluanov, Michael
AU - Hwang, Chaewon
AU - Tam, Jonathan
AU - Khan, Tanya
AU - Morgenstern, Ari
AU - Wiener, Lauren
AU - Vazquez, Juan M.
AU - Zafar, Hiba
AU - Wen, Robert
AU - Muratkalyeva, Malika
AU - Doerig, Katherine
AU - Zagorulya, Maria
AU - Cole, Lauren
AU - Catalano, Sophia
AU - Lobo Ladd, Aliny A.B.
AU - Coppi, A. Augusto
AU - Coskun, Yüksel
AU - Tian, Xiao
AU - Ablaeva, Julia
AU - Nevo, Eviatar
AU - Gladyshev, Vadim N.
AU - Zhang, Zhengdong D.
AU - Vijg, Jan
AU - Seluanov, Andrei
AU - Gorbunova, Vera
N1 - Publisher Copyright:
© Patrick et al.
PY - 2016
Y1 - 2016
N2 - Differences in the way human and mouse fibroblasts experience senescence in culture had long puzzled researchers. While senescence of human cells is mediated by telomere shortening, Parrinello et al. demonstrated that senescence of mouse cells is caused by extreme oxygen sensitivity. It was hypothesized that the striking difference in oxygen sensitivity between mouse and human cells explains their different rates of aging. To test if this hypothesis is broadly applicable, we cultured cells from 16 rodent species with diverse lifespans in 3% and 21% oxygen and compared their growth rates. Unexpectedly, fibroblasts derived from laboratory mouse strains were the only cells demonstrating extreme sensitivity to oxygen. Cells from hamster, muskrat, woodchuck, capybara, blind mole rat, paca, squirrel, beaver, naked mole rat and wild-caught mice were mildly sensitive to oxygen, while cells from rat, gerbil, deer mouse, chipmunk, guinea pig and chinchilla showed no difference in the growth rate between 3% and 21% oxygen. We conclude that, although the growth of primary fibroblasts is generally improved by maintaining cells in 3% oxygen, the extreme oxygen sensitivity is a peculiarity of laboratory mouse strains, possibly related to their very long telomeres, and fibroblast oxygen sensitivity does not directly correlate with species' lifespan.
AB - Differences in the way human and mouse fibroblasts experience senescence in culture had long puzzled researchers. While senescence of human cells is mediated by telomere shortening, Parrinello et al. demonstrated that senescence of mouse cells is caused by extreme oxygen sensitivity. It was hypothesized that the striking difference in oxygen sensitivity between mouse and human cells explains their different rates of aging. To test if this hypothesis is broadly applicable, we cultured cells from 16 rodent species with diverse lifespans in 3% and 21% oxygen and compared their growth rates. Unexpectedly, fibroblasts derived from laboratory mouse strains were the only cells demonstrating extreme sensitivity to oxygen. Cells from hamster, muskrat, woodchuck, capybara, blind mole rat, paca, squirrel, beaver, naked mole rat and wild-caught mice were mildly sensitive to oxygen, while cells from rat, gerbil, deer mouse, chipmunk, guinea pig and chinchilla showed no difference in the growth rate between 3% and 21% oxygen. We conclude that, although the growth of primary fibroblasts is generally improved by maintaining cells in 3% oxygen, the extreme oxygen sensitivity is a peculiarity of laboratory mouse strains, possibly related to their very long telomeres, and fibroblast oxygen sensitivity does not directly correlate with species' lifespan.
KW - Fibroblasts
KW - Human
KW - Oxygen
KW - Rodents
KW - Senescence
UR - http://www.scopus.com/inward/record.url?scp=84976363354&partnerID=8YFLogxK
U2 - 10.18632/aging.100958
DO - 10.18632/aging.100958
M3 - Article
C2 - 27163160
AN - SCOPUS:84976363354
SN - 1945-4589
VL - 8
SP - 841
EP - 847
JO - Aging
JF - Aging
IS - 5
ER -