TY - JOUR
T1 - Selective loss of the transforming growth factor-β apoptotic signaling pathway in mutant NRP-154 rat prostatic epithelial cells
AU - Larisch-Bloch, Sarit
AU - Danielpour, David
AU - Roche, Nanette S.
AU - Lotan, Rona
AU - Hsing, Andrew Y.
AU - Kerner, Hedviga
AU - Hajouj, Taleb
AU - Lechleider, Robert J.
AU - Roberts, Anita B.
PY - 2000/1
Y1 - 2000/1
N2 - Retroviral insertional mutagenesis was used to select mutant NRP-154 rat prostate carcinoma cells resistant to transforming growth factor (TGF)-β- induced cell death. Similar to the parental cells, a mutant clone, M-NRP1, expressed TGF-β receptors and was still responsive to induction both of direct target genes by TGF-β and of apoptosis by staurosporine or okadaic acid. In contrast, indicators of cell growth, strongly suppressed by TGF-β in the parental cells, were unaffected in M-NRP1 cells. M-NRP1 cells overexpress the antiapoptotic protein, Bcl-xL, and show dysregulated expression and localization of a protein related to a novel human septin, ARTS (designation of apoptotic response to TGF-β signals), cloned by homology to an exonic sequence flanked by the viral long terminal repeats in M-NRP1 cells and shown to make cells competent to undergo apoptosis in response to TGF-β. We propose that ARTS might operate within the same apoptotic pathway as Bcl-xL and that M-NRP1 cells could serve as a useful model for characterization of this pathway.
AB - Retroviral insertional mutagenesis was used to select mutant NRP-154 rat prostate carcinoma cells resistant to transforming growth factor (TGF)-β- induced cell death. Similar to the parental cells, a mutant clone, M-NRP1, expressed TGF-β receptors and was still responsive to induction both of direct target genes by TGF-β and of apoptosis by staurosporine or okadaic acid. In contrast, indicators of cell growth, strongly suppressed by TGF-β in the parental cells, were unaffected in M-NRP1 cells. M-NRP1 cells overexpress the antiapoptotic protein, Bcl-xL, and show dysregulated expression and localization of a protein related to a novel human septin, ARTS (designation of apoptotic response to TGF-β signals), cloned by homology to an exonic sequence flanked by the viral long terminal repeats in M-NRP1 cells and shown to make cells competent to undergo apoptosis in response to TGF-β. We propose that ARTS might operate within the same apoptotic pathway as Bcl-xL and that M-NRP1 cells could serve as a useful model for characterization of this pathway.
UR - http://www.scopus.com/inward/record.url?scp=0033974321&partnerID=8YFLogxK
M3 - Article
C2 - 10672898
AN - SCOPUS:0033974321
SN - 1044-9523
VL - 11
SP - 1
EP - 10
JO - Cell Growth and Differentiation
JF - Cell Growth and Differentiation
IS - 1
ER -