Role of melanoma inhibitor of apoptosis (ML-IAP) protein, a member of the baculoviral IAP repeat (BIR) domain family, in the regulation of C-RAF kinase and cell migration

Tripat Kaur Oberoi-Khanuja, Christiaan Karreman, Sarit Larisch, Ulf R. Rapp, Krishnaraj Rajalingam

Research output: Contribution to journalArticlepeer-review

Abstract

Inhibitor of apoptosis (IAPs) proteins are characterized by the presence of evolutionarily conserved baculoviral inhibitor of apoptosis repeat (BIR) domains, predominantly known for their role in inhibiting caspases and, thereby, apoptosis. We have shown previously that multi-BIR domain-containing IAPs, cellular IAPs, and X-linked IAP can control tumor cell migration by directly regulating the protein stability of C-RAF kinase. Here, we extend our observations to a single BIR domain containing IAP family member melanoma-IAP (ML-IAP). We show that ML-IAP can directly bind to C-RAF and that ML-IAP depletion leads to an increase in C-RAF protein levels, MAPK activation, and cell migration in melanoma cells. Thus, our results unveil a thus far unknown role for ML-IAP in controlling C-RAF stability and cell migration.

Original languageEnglish
Pages (from-to)28445-28455
Number of pages11
JournalJournal of Biological Chemistry
Volume287
Issue number34
DOIs
StatePublished - 17 Aug 2012

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Cell Biology

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