RNA Recombination Enhances Adaptability and Is Required for Virus Spread and Virulence

Yinghong Xiao, Igor M. Rouzine, Simone Bianco, Ashley Acevedo, Elizabeth Faul Goldstein, Mikhail Farkov, Leonid Brodsky, Raul Andino

Research output: Contribution to journalArticlepeer-review

Abstract

Mutation and recombination are central processes driving microbial evolution. A high mutation rate fuels adaptation but also generates deleterious mutations. Recombination between two different genomes may resolve this paradox, alleviating effects of clonal interference and purging deleterious mutations. Here we demonstrate that recombination significantly accelerates adaptation and evolution during acute virus infection. We identified a poliovirus recombination determinant within the virus polymerase, mutation of which reduces recombination rates without altering replication fidelity. By generating a panel of variants with distinct mutation rates and recombination ability, we demonstrate that recombination is essential to enrich the population in beneficial mutations and purge it from deleterious mutations. The concerted activities of mutation and recombination are key to virus spread and virulence in infected animals. These findings inform a mathematical model to demonstrate that poliovirus adapts most rapidly at an optimal mutation rate determined by the trade-off between selection and accumulation of detrimental mutations.

Original languageEnglish
Pages (from-to)493-503
Number of pages11
JournalCell Host and Microbe
Volume19
Issue number4
DOIs
StatePublished - 13 Apr 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

ASJC Scopus subject areas

  • Virology
  • Parasitology
  • Microbiology

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