Repeated, Long-Term Cycling of Putative Stem Cells between Niches in a Basal Chordate

Yuval Rinkevich; Ayelet Voskoboynik; Amalia Rosner; Claudette Rabinowitz; Guy Paz; Matan Oren; Jacob Douek; Gilad Alfassi; Elizabeth Moiseeva; Katherine J. Ishizuka; Karla J. Palmeri; Irving L. Weissman; Buki Rinkevich

doi:10.1016/j.devcel.2012.11.010

Repeated, Long-Term Cycling of Putative Stem Cells between Niches in a Basal Chordate

Yuval Rinkevich, Ayelet Voskoboynik, Amalia Rosner, Claudette Rabinowitz, Guy Paz, Matan Oren, Jacob Douek, Gilad Alfassi, Elizabeth Moiseeva, Katherine J. Ishizuka, Karla J. Palmeri, Irving L. Weissman, Buki Rinkevich

Research output: Contribution to journal › Article › peer-review

Abstract

The mechanisms that sustain stem cells are fundamental to tissue maintenance. Here, we identify " cell islands" (CIs) as a niche for putative germ and somatic stem cells in Botryllus schlosseri, a colonial chordate that undergoes weekly cycles of death and regeneration. Cells within CIs express markers associated with germ and somatic stem cells and gene products that implicate CIs as signaling centers for stem cells. Transplantation of CIs induced long-term germline and somatic chimerism, demonstrating self-renewal and pluripotency of CI cells. Cell labeling and in vivo time-lapse imaging of CI cells reveal waves of migrations from degrading CIs into developing buds, contributing to soma and germline development. Knockdown of cadherin, which is highly expressed within CIs, elicited the migration of CI cells to circulation. Piwi knockdown resulted in regeneration arrest. We suggest that repeated trafficking of stem cells allows them to escape constraints imposed by the niche, enabling self-preservation throughout life.

Original language	English
Pages (from-to)	76-88
Number of pages	13
Journal	Developmental Cell
Volume	24
Issue number	1
DOIs	https://doi.org/10.1016/j.devcel.2012.11.010
State	Published - 14 Jan 2013
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank Chris Patton for his invaluable technical advice and imaging. This study was supported by grants from the United States-Israel Bi-National Science Foundation (2003-010 to B.R. and I.L.W.) and from the Israel Academy of Science (68/10 to B.R. and 1342/08 to J.D.) and by National Institutes of Health grants 1R56AI089968-01, RO1GM100315-01, and 1R01AG037968-01 to I.L.W. Y.R. is supported by the Human Frontier Science Program Long Term Fellowship, the Machiah Foundation Fellowship, and the Siebel Foundation (1119368-104-GHBJI).

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.devcel.2012.11.010

Cite this

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abstract = "The mechanisms that sustain stem cells are fundamental to tissue maintenance. Here, we identify {"} cell islands{"} (CIs) as a niche for putative germ and somatic stem cells in Botryllus schlosseri, a colonial chordate that undergoes weekly cycles of death and regeneration. Cells within CIs express markers associated with germ and somatic stem cells and gene products that implicate CIs as signaling centers for stem cells. Transplantation of CIs induced long-term germline and somatic chimerism, demonstrating self-renewal and pluripotency of CI cells. Cell labeling and in vivo time-lapse imaging of CI cells reveal waves of migrations from degrading CIs into developing buds, contributing to soma and germline development. Knockdown of cadherin, which is highly expressed within CIs, elicited the migration of CI cells to circulation. Piwi knockdown resulted in regeneration arrest. We suggest that repeated trafficking of stem cells allows them to escape constraints imposed by the niche, enabling self-preservation throughout life.",

author = "Yuval Rinkevich and Ayelet Voskoboynik and Amalia Rosner and Claudette Rabinowitz and Guy Paz and Matan Oren and Jacob Douek and Gilad Alfassi and Elizabeth Moiseeva and Ishizuka, {Katherine J.} and Palmeri, {Karla J.} and Weissman, {Irving L.} and Buki Rinkevich",

note = "Funding Information: The authors thank Chris Patton for his invaluable technical advice and imaging. This study was supported by grants from the United States-Israel Bi-National Science Foundation (2003-010 to B.R. and I.L.W.) and from the Israel Academy of Science (68/10 to B.R. and 1342/08 to J.D.) and by National Institutes of Health grants 1R56AI089968-01, RO1GM100315-01, and 1R01AG037968-01 to I.L.W. Y.R. is supported by the Human Frontier Science Program Long Term Fellowship, the Machiah Foundation Fellowship, and the Siebel Foundation (1119368-104-GHBJI). ",

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AU - Rabinowitz, Claudette

AU - Paz, Guy

AU - Oren, Matan

AU - Douek, Jacob

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Repeated, Long-Term Cycling of Putative Stem Cells between Niches in a Basal Chordate

Abstract

Bibliographical note

ASJC Scopus subject areas

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