Reduction in spine density associated with long-term potentiation in the dentate gyrus suggests a spine fusion-and-branching model of potentiation

Dmitri A. Rusakov, Gal Richter-Levin, Michael G. Stewart, T. V.P. Bliss

Research output: Contribution to journalArticlepeer-review

Abstract

Approximately 2700 dendritic spines in Golgi-impregnated hippocampal granule cells were quantified via image analysis 24 h after the unilateral induction of long-term potentiation in seven rats. Stereological corrections were made using a tilting disector and analytical unfolding technique. In the potentiated hemisphere the mean spine density along dendrites was reduced by ~ 20%. The relative frequency of shorter, thicker spines was increased in potentiated tissue. Physiological consequences of two morphological changes leading to a reduction in spine density (retraction or fusion of spines) were examined using a compartmental model of a simplified granule cell. The model was constructed in the NEURON modeling environment and included a realistic population of 60 dendritic spines (with dual-component synapses and active Ca2+-dependent mechanisms). Simulations demonstrated that potentiation of postsynaptic responses was compatible with fusion (with branching) of a proportion of spines with their neighbors but was not compatible with retraction of spines. This result held over wide variations of model parameters as long as dendritic membranes were assumed to be excitable.

Original languageEnglish
Pages (from-to)489-500
Number of pages12
JournalHippocampus
Volume7
Issue number5
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • Active membrane
  • Compartmental modeling
  • Dendritic spine
  • Golgi study
  • Morphological changes

ASJC Scopus subject areas

  • Cognitive Neuroscience

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