RAPD divergence caused by microsite edaphic selection in wild barley

Edward D. Owuor; Tzion Fahima; Alex Beharav; Abraham Korol; Eviatar Nevo

doi:10.1023/A:1003781711908

RAPD divergence caused by microsite edaphic selection in wild barley

Edward D. Owuor, Tzion Fahima, Alex Beharav, Abraham Korol, Eviatar Nevo

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to assess genetic diversity in four subpopulations (86 individuals) of wild barley, Hordeum spontaneum, sampled from Tabigha microsite near the Sea of Galilee, Israel. The microsite consists of two 100 m transects that are topographically separated by 100 m, each equally subdivided into 50 m of basalt and terra rossa soil types. Despite the same macroclimate characterizing the area around the Sea of Galilee, the microsite offers two edaphically different microhabitats, with basalt being a more ecologically heterogeneous and broader-niche than the relatively drier but more homogeneous and narrow-niche terra rossa. Analysis of 118 putative loci revealed significant (P < 0.05) genetic differentiation in polymorphism (P_0.05) between the two soils across the transects with P being higher in the more heterogeneous basalt (mean P_0.05 = 0.902), than in terra rossa (mean P_0.05 = 0.820). Gene diversity (He) was higher in basalt (mean He = 0.371), than in terra rossa (mean He = 0.259). Furthermore, unique alleles were confined to one soil type, either in one or both transects. Rare alleles were observed more frequently in terra rossa than basalt, and in transect II only. Gametic phase disequilibria showed a larger multilocus association of alleles in basalt than terra rossa, and in transect I than II. Spearman rank correlation (r(s)) revealed a strong association between specific loci and soil types, and transects. Also, analysis of multilocus organization revealed soil-specific multilocus-genotypes. Therefore, our results suggest an edaphically differentiated genetic structure, which corroborates the niche width-variation hypothesis, and can be explained, in part, by natural selection. This pattern of RAPD diversity is in agreement with allozyme and hordein protein diversities in the same subpopulations studied previously.

Original language	English
Pages (from-to)	177-192
Number of pages	16
Journal	Genetica
Volume	105
Issue number	2
DOIs	https://doi.org/10.1023/A:1003781711908
State	Published - 1999

Bibliographical note

Funding Information:
This work is part of the PhD dissertation of Edward D. Owuor, and is supported by the Authority of Advanced Studies at the University of Haifa, the Israeli Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors would like to express their sincere appreciation to Dr Avigdor Beiles for his important discussions and great contribution to this manuscript, Dr Hallerman E. of the Department of Fisheries and Wildlife Sciences, Virginia Polytechnic Institute and State University, for his contribution and to the anonymous reviewers for their valuable comments.

Keywords

Barley
Hordeum spontaneum
Microsite ecology
Molecular edaphic-differentiation
RAPD

ASJC Scopus subject areas

Animal Science and Zoology
Genetics
Plant Science
Insect Science

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10.1023/A:1003781711908

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@article{b8bc66533928407c8293c5e074dfc57b,

title = "RAPD divergence caused by microsite edaphic selection in wild barley",

abstract = "Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to assess genetic diversity in four subpopulations (86 individuals) of wild barley, Hordeum spontaneum, sampled from Tabigha microsite near the Sea of Galilee, Israel. The microsite consists of two 100 m transects that are topographically separated by 100 m, each equally subdivided into 50 m of basalt and terra rossa soil types. Despite the same macroclimate characterizing the area around the Sea of Galilee, the microsite offers two edaphically different microhabitats, with basalt being a more ecologically heterogeneous and broader-niche than the relatively drier but more homogeneous and narrow-niche terra rossa. Analysis of 118 putative loci revealed significant (P < 0.05) genetic differentiation in polymorphism (P0.05) between the two soils across the transects with P being higher in the more heterogeneous basalt (mean P0.05 = 0.902), than in terra rossa (mean P0.05 = 0.820). Gene diversity (He) was higher in basalt (mean He = 0.371), than in terra rossa (mean He = 0.259). Furthermore, unique alleles were confined to one soil type, either in one or both transects. Rare alleles were observed more frequently in terra rossa than basalt, and in transect II only. Gametic phase disequilibria showed a larger multilocus association of alleles in basalt than terra rossa, and in transect I than II. Spearman rank correlation (r(s)) revealed a strong association between specific loci and soil types, and transects. Also, analysis of multilocus organization revealed soil-specific multilocus-genotypes. Therefore, our results suggest an edaphically differentiated genetic structure, which corroborates the niche width-variation hypothesis, and can be explained, in part, by natural selection. This pattern of RAPD diversity is in agreement with allozyme and hordein protein diversities in the same subpopulations studied previously.",

keywords = "Barley, Hordeum spontaneum, Microsite ecology, Molecular edaphic-differentiation, RAPD",

author = "Owuor, {Edward D.} and Tzion Fahima and Alex Beharav and Abraham Korol and Eviatar Nevo",

note = "Funding Information: This work is part of the PhD dissertation of Edward D. Owuor, and is supported by the Authority of Advanced Studies at the University of Haifa, the Israeli Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors would like to express their sincere appreciation to Dr Avigdor Beiles for his important discussions and great contribution to this manuscript, Dr Hallerman E. of the Department of Fisheries and Wildlife Sciences, Virginia Polytechnic Institute and State University, for his contribution and to the anonymous reviewers for their valuable comments.",

year = "1999",

doi = "10.1023/A:1003781711908",

language = "English",

volume = "105",

pages = "177--192",

journal = "Genetica",

issn = "0016-6707",

publisher = "Springer Netherlands",

number = "2",

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T1 - RAPD divergence caused by microsite edaphic selection in wild barley

AU - Owuor, Edward D.

AU - Fahima, Tzion

AU - Beharav, Alex

AU - Korol, Abraham

AU - Nevo, Eviatar

N1 - Funding Information: This work is part of the PhD dissertation of Edward D. Owuor, and is supported by the Authority of Advanced Studies at the University of Haifa, the Israeli Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors would like to express their sincere appreciation to Dr Avigdor Beiles for his important discussions and great contribution to this manuscript, Dr Hallerman E. of the Department of Fisheries and Wildlife Sciences, Virginia Polytechnic Institute and State University, for his contribution and to the anonymous reviewers for their valuable comments.

PY - 1999

Y1 - 1999

N2 - Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to assess genetic diversity in four subpopulations (86 individuals) of wild barley, Hordeum spontaneum, sampled from Tabigha microsite near the Sea of Galilee, Israel. The microsite consists of two 100 m transects that are topographically separated by 100 m, each equally subdivided into 50 m of basalt and terra rossa soil types. Despite the same macroclimate characterizing the area around the Sea of Galilee, the microsite offers two edaphically different microhabitats, with basalt being a more ecologically heterogeneous and broader-niche than the relatively drier but more homogeneous and narrow-niche terra rossa. Analysis of 118 putative loci revealed significant (P < 0.05) genetic differentiation in polymorphism (P0.05) between the two soils across the transects with P being higher in the more heterogeneous basalt (mean P0.05 = 0.902), than in terra rossa (mean P0.05 = 0.820). Gene diversity (He) was higher in basalt (mean He = 0.371), than in terra rossa (mean He = 0.259). Furthermore, unique alleles were confined to one soil type, either in one or both transects. Rare alleles were observed more frequently in terra rossa than basalt, and in transect II only. Gametic phase disequilibria showed a larger multilocus association of alleles in basalt than terra rossa, and in transect I than II. Spearman rank correlation (r(s)) revealed a strong association between specific loci and soil types, and transects. Also, analysis of multilocus organization revealed soil-specific multilocus-genotypes. Therefore, our results suggest an edaphically differentiated genetic structure, which corroborates the niche width-variation hypothesis, and can be explained, in part, by natural selection. This pattern of RAPD diversity is in agreement with allozyme and hordein protein diversities in the same subpopulations studied previously.

AB - Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to assess genetic diversity in four subpopulations (86 individuals) of wild barley, Hordeum spontaneum, sampled from Tabigha microsite near the Sea of Galilee, Israel. The microsite consists of two 100 m transects that are topographically separated by 100 m, each equally subdivided into 50 m of basalt and terra rossa soil types. Despite the same macroclimate characterizing the area around the Sea of Galilee, the microsite offers two edaphically different microhabitats, with basalt being a more ecologically heterogeneous and broader-niche than the relatively drier but more homogeneous and narrow-niche terra rossa. Analysis of 118 putative loci revealed significant (P < 0.05) genetic differentiation in polymorphism (P0.05) between the two soils across the transects with P being higher in the more heterogeneous basalt (mean P0.05 = 0.902), than in terra rossa (mean P0.05 = 0.820). Gene diversity (He) was higher in basalt (mean He = 0.371), than in terra rossa (mean He = 0.259). Furthermore, unique alleles were confined to one soil type, either in one or both transects. Rare alleles were observed more frequently in terra rossa than basalt, and in transect II only. Gametic phase disequilibria showed a larger multilocus association of alleles in basalt than terra rossa, and in transect I than II. Spearman rank correlation (r(s)) revealed a strong association between specific loci and soil types, and transects. Also, analysis of multilocus organization revealed soil-specific multilocus-genotypes. Therefore, our results suggest an edaphically differentiated genetic structure, which corroborates the niche width-variation hypothesis, and can be explained, in part, by natural selection. This pattern of RAPD diversity is in agreement with allozyme and hordein protein diversities in the same subpopulations studied previously.

KW - Barley

KW - Hordeum spontaneum

KW - Microsite ecology

KW - Molecular edaphic-differentiation

KW - RAPD

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DO - 10.1023/A:1003781711908

M3 - Article

AN - SCOPUS:0032756975

SN - 0016-6707

VL - 105

SP - 177

EP - 192

JO - Genetica

JF - Genetica

IS - 2

ER -

RAPD divergence caused by microsite edaphic selection in wild barley

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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