Quantification of bacterial adhesion to tissue in high-Throughput kinetics

Nimrod Shteindel, Danielle Gutman, Gil Atzmon, Yoram Gerchman

Research output: Contribution to journalArticlepeer-review

Abstract

Bacterial adhesion to tissue is the starting point for many pathogenic processes and beneficial interactions. The dynamics and speed of adhesion (minutes) make high-resolution temporal kinetic data important, but this capability is absent from the current toolset. We present a high-Throughput method with a second-To-minute kinetic resolution, testing the adhesion of Pseudomonas aeruginosa PAO1 wild-Type, flagella-, pili-, and quorum-sensing mutants to human embryonic kidney (HEK293) cells. Adhesion rates were in good correlation with HEK293 confluence, and the ways in which various bacterial mutations modified adhesion patterns are in agreement with the published literature. This simple assay can facilitate drug screening and treatment development as well as provide a better understanding of the interactions of pathogenic and probiotic bacteria with tissues, allowing the design of interventions and prevention treatments.

Original languageEnglish
Article numberbpad014
JournalBiology Methods and Protocols
Volume8
Issue number1
DOIs
StatePublished - 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press.

Keywords

  • Pseudomonas aeruginosa
  • adhesion of bacteria to tissue
  • high throughput
  • kinetics

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General Biochemistry, Genetics and Molecular Biology

Fingerprint

Dive into the research topics of 'Quantification of bacterial adhesion to tissue in high-Throughput kinetics'. Together they form a unique fingerprint.

Cite this