Pyridine-type alkaloid composition affects bacterial community composition of floral nectar

Yana Aizenberg-Gershtein; Ido Izhaki; Rakesh Santhanam; Pavan Kumar; Ian T. Baldwin; Malka Halpern

doi:10.1038/srep11536

Pyridine-type alkaloid composition affects bacterial community composition of floral nectar

Yana Aizenberg-Gershtein, Ido Izhaki, Rakesh Santhanam, Pavan Kumar, Ian T. Baldwin, Malka Halpern

Research output: Contribution to journal › Article › peer-review

Abstract

Pyridine-type alkaloids are most common in Nicotiana species. To study the effect of alkaloid composition on bacterial community composition in floral nectar, we compared the nicotine-rich wild type (WT) N. attenuata, the nicotine biosynthesis-silenced N. attenuata that was rich in anatabine and the anabasine-rich WT N. glauca plants. We found that the composition of these secondary metabolites in the floral nectar drastically affected the bacterial community richness, diversity and composition. Significant differences were found between the bacterial community compositions in the nectar of the three plants with a much greater species richness and diversity in the nectar from the transgenic plant. The highest community composition similarity index was detected between the two wild type plants. The different microbiome composition and diversity, caused by the different pyridine-type alkaloid composition, could modify the nutritional content of the nectar and consequently, may contribute to the change in the nectar consumption and visitation. These may indirectly have an effect on plant fitness.

Original language	English
Article number	11536
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep11536
State	Published - 30 Jun 2015

Bibliographical note

Funding Information:
This study was supported by grants from the Israel Science Foundation (ISF, grants no. 189/08 and 1094/12), the Max Planck Society and the European Research Council advanced grant, ClockworkGreen (an advanced ERC grant, ClockworkGreen, 293926). We thank Eva Rothe, Matthias Schoettner and Ting Yang for their help in the nectar analysis.

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title = "Pyridine-type alkaloid composition affects bacterial community composition of floral nectar",

abstract = "Pyridine-type alkaloids are most common in Nicotiana species. To study the effect of alkaloid composition on bacterial community composition in floral nectar, we compared the nicotine-rich wild type (WT) N. attenuata, the nicotine biosynthesis-silenced N. attenuata that was rich in anatabine and the anabasine-rich WT N. glauca plants. We found that the composition of these secondary metabolites in the floral nectar drastically affected the bacterial community richness, diversity and composition. Significant differences were found between the bacterial community compositions in the nectar of the three plants with a much greater species richness and diversity in the nectar from the transgenic plant. The highest community composition similarity index was detected between the two wild type plants. The different microbiome composition and diversity, caused by the different pyridine-type alkaloid composition, could modify the nutritional content of the nectar and consequently, may contribute to the change in the nectar consumption and visitation. These may indirectly have an effect on plant fitness.",

author = "Yana Aizenberg-Gershtein and Ido Izhaki and Rakesh Santhanam and Pavan Kumar and Baldwin, {Ian T.} and Malka Halpern",

note = "Funding Information: This study was supported by grants from the Israel Science Foundation (ISF, grants no. 189/08 and 1094/12), the Max Planck Society and the European Research Council advanced grant, ClockworkGreen (an advanced ERC grant, ClockworkGreen, 293926). We thank Eva Rothe, Matthias Schoettner and Ting Yang for their help in the nectar analysis.",

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AU - Santhanam, Rakesh

AU - Kumar, Pavan

AU - Baldwin, Ian T.

AU - Halpern, Malka

N1 - Funding Information: This study was supported by grants from the Israel Science Foundation (ISF, grants no. 189/08 and 1094/12), the Max Planck Society and the European Research Council advanced grant, ClockworkGreen (an advanced ERC grant, ClockworkGreen, 293926). We thank Eva Rothe, Matthias Schoettner and Ting Yang for their help in the nectar analysis.

PY - 2015/6/30

Y1 - 2015/6/30

N2 - Pyridine-type alkaloids are most common in Nicotiana species. To study the effect of alkaloid composition on bacterial community composition in floral nectar, we compared the nicotine-rich wild type (WT) N. attenuata, the nicotine biosynthesis-silenced N. attenuata that was rich in anatabine and the anabasine-rich WT N. glauca plants. We found that the composition of these secondary metabolites in the floral nectar drastically affected the bacterial community richness, diversity and composition. Significant differences were found between the bacterial community compositions in the nectar of the three plants with a much greater species richness and diversity in the nectar from the transgenic plant. The highest community composition similarity index was detected between the two wild type plants. The different microbiome composition and diversity, caused by the different pyridine-type alkaloid composition, could modify the nutritional content of the nectar and consequently, may contribute to the change in the nectar consumption and visitation. These may indirectly have an effect on plant fitness.

AB - Pyridine-type alkaloids are most common in Nicotiana species. To study the effect of alkaloid composition on bacterial community composition in floral nectar, we compared the nicotine-rich wild type (WT) N. attenuata, the nicotine biosynthesis-silenced N. attenuata that was rich in anatabine and the anabasine-rich WT N. glauca plants. We found that the composition of these secondary metabolites in the floral nectar drastically affected the bacterial community richness, diversity and composition. Significant differences were found between the bacterial community compositions in the nectar of the three plants with a much greater species richness and diversity in the nectar from the transgenic plant. The highest community composition similarity index was detected between the two wild type plants. The different microbiome composition and diversity, caused by the different pyridine-type alkaloid composition, could modify the nutritional content of the nectar and consequently, may contribute to the change in the nectar consumption and visitation. These may indirectly have an effect on plant fitness.

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Pyridine-type alkaloid composition affects bacterial community composition of floral nectar

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