Purification and particular characterization of laccase from the Ling Zhi or Reishi medicinal mushroom Ganoderma lucidum (W. Curt. Fr.) P. Karst. 447 (Aphyllophoromycetideae)

George G. Songulashvili, Vladimir Elisashvili, Solomon P. Wasser, Eviatar Nevo, Yitzhak Hadar

Research output: Contribution to journalArticlepeer-review

Abstract

The Ganoderma lucidum strain 447 was cultivated in a 10-L fermentor. We used the ethanol-production residue (by wheat) (REP) as the growth substrate and Cu as an inductor. Ganoderma lucidum was grown for 8 days before laccase reached the highest yield (188,600 U L-1). The purification enzyme appeared as two-laccase isozyme bands on SDS-PAGE. The molecular masses were 43 and 56 kDa by SDS-PAGE. The pH optimum for 2,2'-azino-bis-[3-ethyltiazoline-6-sulfonate] (ABTS) oxidation was 3 in citric/acetic buffers. In this study, the optimum temperature for laccase activity was determined to be 30°C. The kinetic of laccase was experimented on using 12 phenolic substrates. The lowest Km values (0.0048 and 0.005 mM) were found for syringaldazine and ABTS, respectively.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalInternational Journal of Medicinal Mushrooms
Volume10
Issue number4
DOIs
StatePublished - 2008

Keywords

  • Characterization
  • Enzyme kinetic
  • Ganoderma lucidum
  • Laccase
  • Ling Zhi or Reishi mushroom
  • Medicinal mushroom
  • Purifi cation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Pharmacology
  • Drug Discovery

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