Abstract
The Ganoderma lucidum strain 447 was cultivated in a 10-L fermentor. We used the ethanol-production residue (by wheat) (REP) as the growth substrate and Cu as an inductor. Ganoderma lucidum was grown for 8 days before laccase reached the highest yield (188,600 U L-1). The purification enzyme appeared as two-laccase isozyme bands on SDS-PAGE. The molecular masses were 43 and 56 kDa by SDS-PAGE. The pH optimum for 2,2'-azino-bis-[3-ethyltiazoline-6-sulfonate] (ABTS) oxidation was 3 in citric/acetic buffers. In this study, the optimum temperature for laccase activity was determined to be 30°C. The kinetic of laccase was experimented on using 12 phenolic substrates. The lowest Km values (0.0048 and 0.005 mM) were found for syringaldazine and ABTS, respectively.
Original language | English |
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Pages (from-to) | 361-368 |
Number of pages | 8 |
Journal | International Journal of Medicinal Mushrooms |
Volume | 10 |
Issue number | 4 |
DOIs | |
State | Published - 2008 |
Keywords
- Characterization
- Enzyme kinetic
- Ganoderma lucidum
- Laccase
- Ling Zhi or Reishi mushroom
- Medicinal mushroom
- Purifi cation
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Pharmacology
- Drug Discovery