Pronounced cancer resistance in a subterranean rodent, the blind mole-rat, Spalax: In vivo and in vitro evidence

Irena Manov, Mark Hirsh, Theodore C. Iancu, Assaf Malik, Nick Sotnichenko, Mark Band, Aaron Avivi, Imad Shams

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Subterranean blind mole rats (Spalax) are hypoxia tolerant (down to 3% O2), long lived (>20 years) rodents showing no clear signs of aging or aging related disorders. In 50 years of Spalax research, spontaneous tumors have never been recorded among thousands of individuals. Here we addressed the questions of (1) whether Spalax is resistant to chemically-induced tumorigenesis, and (2) whether normal fibroblasts isolated from Spalax possess tumor-suppressive activity.Results: Treating animals with 3-Methylcholantrene (3MCA) and 7,12-Dimethylbenz(a) anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA), two potent carcinogens, confirmed Spalax high resistance to chemically induced cancers. While all mice and rats developed the expected tumors following treatment with both carcinogens, among Spalax no tumors were observed after DMBA/TPA treatment, while 3MCA induced benign fibroblastic proliferation in 2 Spalax individuals out of12, and only a single animal from the advanced age group developed malignancy 18 months post-treatment. The remaining animals are still healthy 30 months post-treatment. In vitro experiments showed an extraordinary ability of normal Spalax cultured fibroblasts to restrict malignant behavior in a broad spectrum of human-derived and in newly isolated Spalax 3MCA-induced cancer cell lines. Growth of cancer cells was inhibited by either direct interaction with Spalax fibroblasts or with soluble factors released into culture media and soft agar. This was accompanied by decreased cancer cell viability, reduced colony formation in soft agar, disturbed cell cycle progression, chromatin condensation and mitochondrial fragmentation. Cells from another cancer resistant subterranean mammal, the naked mole rat, were also tested for direct effect on cancer cells and, similar to Spalax, demonstrated anti-cancer activity. No effect on cancer cells was observed using fibroblasts from mouse, rat or Acomys. Spalax fibroblast conditioned media had no effect on proliferation of noncancerous cells.Conclusions: This report provides pioneering evidence that Spalax is not only resistant to spontaneous cancer but also to experimentally induced cancer, and shows the unique ability of Spalax normal fibroblasts to inhibit growth and kill cancer cells, but not normal cells, either through direct fibroblast-cancer cell interaction or via soluble factors. Obviously, along with adaptation to hypoxia, Spalax has evolved efficient anti-cancer mechanisms yet to be elucidated. Exploring the molecular mechanisms allowing Spalax to survive in extreme environments and to escape cancer as well as to kill homologous and heterologous cancer cells may hold the key for understanding the molecular nature of host resistance to cancer and identify new anti-cancer strategies for treating humans.

Original languageEnglish
Article number91
JournalBMC Biology
Volume11
DOIs
StatePublished - 9 Aug 2013

Bibliographical note

Funding Information:
This study was partially supported by Israel Cancer Association (ICA) grant (# 20130065) to AA and MH, and by The Caesarea Edmond Benjamin de Rothschild Foundation Institute for Interdisciplinary Science (C.R.I.) to IS. IM was supported by the Ministry of Immigration Absorption and the Committee for Planning and Budgeting of the Council for Higher Education, Israel, within the framework of the KAMEA program, and, in part, by Dan David Foundation. We wish to thank Mrs. Alma Joel, Mrs. Yulia Pollak, Dr. Sagie Schif-Zuck and Dr. Olga Raskina for their assistance, Dr. Nili Avni-Magen for supplying the Heterocephalus, and Prof. Abraham Korol for his dedicated support and fruitful discussions.

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • Ecology, Evolution, Behavior and Systematics
  • General Biochemistry, Genetics and Molecular Biology
  • Structural Biology
  • Physiology
  • Biotechnology
  • Plant Science
  • Cell Biology
  • Developmental Biology

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