Proline transport into brush border membrane vesicles from the midgut of Manduca sexta larvae

M. Reuveni; P. E. Dunn

doi:10.1016/0300-9629(94)90370-0

Proline transport into brush border membrane vesicles from the midgut of Manduca sexta larvae

Research output: Contribution to journal › Article › peer-review

Abstract

The unidirectional transport of proline by midgut epithelium cells of the tobacco hornworm, Manduca sexta, was investigated in brush border membrane vesicles. Both K.⁺-stimulated and K⁺-insensitive transport pathways were identified. Analyses of K⁺-dependent proline transport revealed 1:1 ratio of K⁺ to proline, a K_m of 13 mM for K⁺ and a decrease in both K_m (from 18 mM to 3mM) and V_max (from 37 nmol/mg protein/min to 10 nmol/mg protein/min) for proline in the presence of a K⁺ gradient. The profiles of cis-inhibition by other amino acids demonstrated that proline is transported into midgut cells by a transport system that is shared by other neutral amino acids.

Original language	English
Pages (from-to)	685-691
Number of pages	7
Journal	Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume	107
Issue number	4
DOIs	https://doi.org/10.1016/0300-9629(94)90370-0
State	Published - Apr 1994
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgemenfs-The authors thank Professors J. J. Neal, J. Stuart and C. Y. Oseto for critical review of the manuscript. This research was supported by United States Department of Agriculture Competitive Research Grant No. 88-37153-4098 to A. I. Aronson and P.E.D. This is Journal Paper No 13,630 from the Indiana Agricultural Experiment Station.

Keywords

Brush border membrane vesicle
Manduca sexta
Proline transport

ASJC Scopus subject areas

Physiology

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10.1016/0300-9629(94)90370-0

Cite this

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title = "Proline transport into brush border membrane vesicles from the midgut of Manduca sexta larvae",

abstract = "The unidirectional transport of proline by midgut epithelium cells of the tobacco hornworm, Manduca sexta, was investigated in brush border membrane vesicles. Both K.+-stimulated and K+-insensitive transport pathways were identified. Analyses of K+-dependent proline transport revealed 1:1 ratio of K+ to proline, a Km of 13 mM for K+ and a decrease in both Km (from 18 mM to 3mM) and Vmax (from 37 nmol/mg protein/min to 10 nmol/mg protein/min) for proline in the presence of a K+ gradient. The profiles of cis-inhibition by other amino acids demonstrated that proline is transported into midgut cells by a transport system that is shared by other neutral amino acids.",

keywords = "Brush border membrane vesicle, Manduca sexta, Proline transport",

author = "M. Reuveni and Dunn, {P. E.}",

note = "Funding Information: Acknowledgemenfs-The authors thank Professors J. J. Neal, J. Stuart and C. Y. Oseto for critical review of the manuscript. This research was supported by United States Department of Agriculture Competitive Research Grant No. 88-37153-4098 to A. I. Aronson and P.E.D. This is Journal Paper No 13,630 from the Indiana Agricultural Experiment Station.",

year = "1994",

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doi = "10.1016/0300-9629(94)90370-0",

language = "English",

volume = "107",

pages = "685--691",

journal = "Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology",

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AU - Reuveni, M.

AU - Dunn, P. E.

N1 - Funding Information: Acknowledgemenfs-The authors thank Professors J. J. Neal, J. Stuart and C. Y. Oseto for critical review of the manuscript. This research was supported by United States Department of Agriculture Competitive Research Grant No. 88-37153-4098 to A. I. Aronson and P.E.D. This is Journal Paper No 13,630 from the Indiana Agricultural Experiment Station.

PY - 1994/4

Y1 - 1994/4

N2 - The unidirectional transport of proline by midgut epithelium cells of the tobacco hornworm, Manduca sexta, was investigated in brush border membrane vesicles. Both K.+-stimulated and K+-insensitive transport pathways were identified. Analyses of K+-dependent proline transport revealed 1:1 ratio of K+ to proline, a Km of 13 mM for K+ and a decrease in both Km (from 18 mM to 3mM) and Vmax (from 37 nmol/mg protein/min to 10 nmol/mg protein/min) for proline in the presence of a K+ gradient. The profiles of cis-inhibition by other amino acids demonstrated that proline is transported into midgut cells by a transport system that is shared by other neutral amino acids.

AB - The unidirectional transport of proline by midgut epithelium cells of the tobacco hornworm, Manduca sexta, was investigated in brush border membrane vesicles. Both K.+-stimulated and K+-insensitive transport pathways were identified. Analyses of K+-dependent proline transport revealed 1:1 ratio of K+ to proline, a Km of 13 mM for K+ and a decrease in both Km (from 18 mM to 3mM) and Vmax (from 37 nmol/mg protein/min to 10 nmol/mg protein/min) for proline in the presence of a K+ gradient. The profiles of cis-inhibition by other amino acids demonstrated that proline is transported into midgut cells by a transport system that is shared by other neutral amino acids.

KW - Brush border membrane vesicle

KW - Manduca sexta

KW - Proline transport

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SN - 0300-9629

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SP - 685

EP - 691

JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

IS - 4

ER -

Proline transport into brush border membrane vesicles from the midgut of Manduca sexta larvae

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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