Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS

Kwadwo Gyapong Agyenim-Boateng; Shengrui Zhang; Md Shariful Islam; Yongzhe Gu; Bin Li; Muhammad Azam; Ahmed M. Abdelghany; Jie Qi; Suprio Ghosh; Abdulwahab S. Shaibu; Berhane Sibhatu Gebregziabher; Yue Feng; Jing Li; Yinghui Li; Chunyi Zhang; Lijuan Qiu; Zhangxiong Liu; Qiuju Liang; Junming Sun

doi:10.1016/j.foodchem.2022.132520

Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS

Kwadwo Gyapong Agyenim-Boateng, Shengrui Zhang, Md Shariful Islam, Yongzhe Gu, Bin Li, Muhammad Azam, Ahmed M. Abdelghany, Jie Qi, Suprio Ghosh, Abdulwahab S. Shaibu, Berhane Sibhatu Gebregziabher, Yue Feng, Jing Li, Yinghui Li, Chunyi Zhang, Lijuan Qiu, Zhangxiong Liu, Qiuju Liang, Junming Sun

Research output: Contribution to journal › Article › peer-review

Abstract

Soybean is a rich source of folates. We optimised the extraction and detection of folates from soybean seeds by HPLC-MS/MS and analysed the folate content and composition of 1074 accessions. Total folate content ranged from 64.51 to 691.24 μg/100 g fresh weight, with 10-fold variation, and 60 elite accessions with over 400 μg/100 g of total folate were identified. The most abundant component was 5-CHO-H₄folate, which accounted for an average of 60% of total folate content. Seed-coat colour, seed weight, ecoregion, and accession type significantly affected soybean folate content. Furthermore, 5-CH₃-H₄folate correlated positively with seed protein (r = 0.24***) and negatively with oil (r = -0.26***). The geographical distribution of folate according to accession origin revealed that accessions from Northeast China contain higher amounts of total folate and 5-CHO-H₄folate. This study provides comprehensive and novel insights into the folate profile of soybean, which will benefit soybean breeding for folate enhancement.

Original language	English
Article number	132520
Journal	Food Chemistry
Volume	384
DOIs	https://doi.org/10.1016/j.foodchem.2022.132520
State	Published - 1 Aug 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

10-Formyl-folic acid (PubChem CID: 135405023)
5,10-Methenyl-tetrahydrofolate (PubChem CID: 135398657)
5-Formyl-tetrahydrofolate (PubChem CID: 135403648)
5-Methyl-tetrahydrofolate (PubChem CID: 135483998)
Acetonitrile (PubChem CID: 6342)
Dihydrofolate (PubChem CID: 135398604)
Elite accessions
Folate
Folic acid (PubChem CID: 135398658)
Formic acid (PubChem CID: 284)
Germplasm
HPLC-MS/MS
Methotrexate (PubChem CID: 126941)
Natural variation
Sodium ascorbate (PubChem CID: 23667548)
Sodium phosphate dibasic (PubChem CID: 24203)
Sodium phosphate monobasic (PubChem CID: 23672064)
Soybean (Glycine max L. Merrill)
Tetrahydrofolate (PubChem CID: 135444742)
α-Amylase (PubChem CID: 62698)
β-Mercaptoethanol (PubChem CID: 1567)

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2022.132520

Cite this

Agyenim-Boateng, K. G., Zhang, S., Islam, M. S., Gu, Y., Li, B., Azam, M., Abdelghany, A. M., Qi, J., Ghosh, S., Shaibu, A. S., Gebregziabher, B. S., Feng, Y., Li, J., Li, Y., Zhang, C., Qiu, L., Liu, Z., Liang, Q., & Sun, J. (2022). Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS. Food Chemistry, 384, Article 132520. https://doi.org/10.1016/j.foodchem.2022.132520

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title = "Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS",

abstract = "Soybean is a rich source of folates. We optimised the extraction and detection of folates from soybean seeds by HPLC-MS/MS and analysed the folate content and composition of 1074 accessions. Total folate content ranged from 64.51 to 691.24 μg/100 g fresh weight, with 10-fold variation, and 60 elite accessions with over 400 μg/100 g of total folate were identified. The most abundant component was 5-CHO-H4folate, which accounted for an average of 60% of total folate content. Seed-coat colour, seed weight, ecoregion, and accession type significantly affected soybean folate content. Furthermore, 5-CH3-H4folate correlated positively with seed protein (r = 0.24***) and negatively with oil (r = -0.26***). The geographical distribution of folate according to accession origin revealed that accessions from Northeast China contain higher amounts of total folate and 5-CHO-H4folate. This study provides comprehensive and novel insights into the folate profile of soybean, which will benefit soybean breeding for folate enhancement.",

keywords = "10-Formyl-folic acid (PubChem CID: 135405023), 5,10-Methenyl-tetrahydrofolate (PubChem CID: 135398657), 5-Formyl-tetrahydrofolate (PubChem CID: 135403648), 5-Methyl-tetrahydrofolate (PubChem CID: 135483998), Acetonitrile (PubChem CID: 6342), Dihydrofolate (PubChem CID: 135398604), Elite accessions, Folate, Folic acid (PubChem CID: 135398658), Formic acid (PubChem CID: 284), Germplasm, HPLC-MS/MS, Methotrexate (PubChem CID: 126941), Natural variation, Sodium ascorbate (PubChem CID: 23667548), Sodium phosphate dibasic (PubChem CID: 24203), Sodium phosphate monobasic (PubChem CID: 23672064), Soybean (Glycine max L. Merrill), Tetrahydrofolate (PubChem CID: 135444742), α-Amylase (PubChem CID: 62698), β-Mercaptoethanol (PubChem CID: 1567)",

author = "Agyenim-Boateng, {Kwadwo Gyapong} and Shengrui Zhang and Islam, {Md Shariful} and Yongzhe Gu and Bin Li and Muhammad Azam and Abdelghany, {Ahmed M.} and Jie Qi and Suprio Ghosh and Shaibu, {Abdulwahab S.} and Gebregziabher, {Berhane Sibhatu} and Yue Feng and Jing Li and Yinghui Li and Chunyi Zhang and Lijuan Qiu and Zhangxiong Liu and Qiuju Liang and Junming Sun",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

year = "2022",

month = aug,

day = "1",

doi = "10.1016/j.foodchem.2022.132520",

language = "English",

volume = "384",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier",

}

Agyenim-Boateng, KG, Zhang, S, Islam, MS, Gu, Y, Li, B, Azam, M, Abdelghany, AM, Qi, J, Ghosh, S, Shaibu, AS, Gebregziabher, BS, Feng, Y, Li, J, Li, Y, Zhang, C, Qiu, L, Liu, Z, Liang, Q & Sun, J 2022, 'Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS', Food Chemistry, vol. 384, 132520. https://doi.org/10.1016/j.foodchem.2022.132520

TY - JOUR

T1 - Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS

AU - Agyenim-Boateng, Kwadwo Gyapong

AU - Zhang, Shengrui

AU - Islam, Md Shariful

AU - Gu, Yongzhe

AU - Li, Bin

AU - Azam, Muhammad

AU - Abdelghany, Ahmed M.

AU - Qi, Jie

AU - Ghosh, Suprio

AU - Shaibu, Abdulwahab S.

AU - Gebregziabher, Berhane Sibhatu

AU - Feng, Yue

AU - Li, Jing

AU - Li, Yinghui

AU - Zhang, Chunyi

AU - Qiu, Lijuan

AU - Liu, Zhangxiong

AU - Liang, Qiuju

AU - Sun, Junming

PY - 2022/8/1

Y1 - 2022/8/1

N2 - Soybean is a rich source of folates. We optimised the extraction and detection of folates from soybean seeds by HPLC-MS/MS and analysed the folate content and composition of 1074 accessions. Total folate content ranged from 64.51 to 691.24 μg/100 g fresh weight, with 10-fold variation, and 60 elite accessions with over 400 μg/100 g of total folate were identified. The most abundant component was 5-CHO-H4folate, which accounted for an average of 60% of total folate content. Seed-coat colour, seed weight, ecoregion, and accession type significantly affected soybean folate content. Furthermore, 5-CH3-H4folate correlated positively with seed protein (r = 0.24***) and negatively with oil (r = -0.26***). The geographical distribution of folate according to accession origin revealed that accessions from Northeast China contain higher amounts of total folate and 5-CHO-H4folate. This study provides comprehensive and novel insights into the folate profile of soybean, which will benefit soybean breeding for folate enhancement.

AB - Soybean is a rich source of folates. We optimised the extraction and detection of folates from soybean seeds by HPLC-MS/MS and analysed the folate content and composition of 1074 accessions. Total folate content ranged from 64.51 to 691.24 μg/100 g fresh weight, with 10-fold variation, and 60 elite accessions with over 400 μg/100 g of total folate were identified. The most abundant component was 5-CHO-H4folate, which accounted for an average of 60% of total folate content. Seed-coat colour, seed weight, ecoregion, and accession type significantly affected soybean folate content. Furthermore, 5-CH3-H4folate correlated positively with seed protein (r = 0.24***) and negatively with oil (r = -0.26***). The geographical distribution of folate according to accession origin revealed that accessions from Northeast China contain higher amounts of total folate and 5-CHO-H4folate. This study provides comprehensive and novel insights into the folate profile of soybean, which will benefit soybean breeding for folate enhancement.

KW - 10-Formyl-folic acid (PubChem CID: 135405023)

KW - 5,10-Methenyl-tetrahydrofolate (PubChem CID: 135398657)

KW - 5-Formyl-tetrahydrofolate (PubChem CID: 135403648)

KW - 5-Methyl-tetrahydrofolate (PubChem CID: 135483998)

KW - Acetonitrile (PubChem CID: 6342)

KW - Dihydrofolate (PubChem CID: 135398604)

KW - Elite accessions

KW - Folate

KW - Folic acid (PubChem CID: 135398658)

KW - Formic acid (PubChem CID: 284)

KW - Germplasm

KW - HPLC-MS/MS

KW - Methotrexate (PubChem CID: 126941)

KW - Natural variation

KW - Sodium ascorbate (PubChem CID: 23667548)

KW - Sodium phosphate dibasic (PubChem CID: 24203)

KW - Sodium phosphate monobasic (PubChem CID: 23672064)

KW - Soybean (Glycine max L. Merrill)

KW - Tetrahydrofolate (PubChem CID: 135444742)

KW - α-Amylase (PubChem CID: 62698)

KW - β-Mercaptoethanol (PubChem CID: 1567)

UR - http://www.scopus.com/inward/record.url?scp=85124979317&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2022.132520

DO - 10.1016/j.foodchem.2022.132520

M3 - Article

C2 - 35217465

AN - SCOPUS:85124979317

SN - 0308-8146

VL - 384

JO - Food Chemistry

JF - Food Chemistry

M1 - 132520

ER -

Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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