TY - CHAP
T1 - Probabilistic methods in directed evolution
T2 - Library size, mutation rate, and diversity
AU - Nov, Yuval
PY - 2014
Y1 - 2014
N2 - Directed evolution has emerged as an important tool for engineering proteins with improved or novel properties. Because of their inherent reliance on randomness, directed evolution protocols are amenable to probabilistic modeling and analysis. This chapter summarizes and reviews in a nonmathematical way some of the probabilistic works related to directed evolution, with particular focus on three of the most widely used methods: saturation mutagenesis, error-prone PCR, and in vitro recombination. The ultimate aim is to provide the reader with practical information to guide the planning and design of directed evolution studies. Importantly, the applications and locations of freely available computational resources to assist with this process are described in detail.
AB - Directed evolution has emerged as an important tool for engineering proteins with improved or novel properties. Because of their inherent reliance on randomness, directed evolution protocols are amenable to probabilistic modeling and analysis. This chapter summarizes and reviews in a nonmathematical way some of the probabilistic works related to directed evolution, with particular focus on three of the most widely used methods: saturation mutagenesis, error-prone PCR, and in vitro recombination. The ultimate aim is to provide the reader with practical information to guide the planning and design of directed evolution studies. Importantly, the applications and locations of freely available computational resources to assist with this process are described in detail.
KW - Computational tools
KW - DNA shuffling
KW - Directed evolution
KW - Error-prone PCR
KW - Library generation
KW - Probabilistic modeling
KW - Saturation mutagenesis
KW - Staggered extension process
UR - http://www.scopus.com/inward/record.url?scp=84925884241&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-1053-3_18
DO - 10.1007/978-1-4939-1053-3_18
M3 - Chapter
C2 - 25055784
AN - SCOPUS:84925884241
SN - 9781493910526
T3 - Methods in Molecular Biology
SP - 261
EP - 278
BT - Directed Evolution Library Creation
PB - Humana Press Inc.
ER -