Pomegranate (Punica granatum) seed linolenic acid isomers were evaluated as selective estrogen receptor modulators (SERMs) in vitro. Punicic acid (PA) inhibited (IC50) estrogen receptor (ER) α at 7.2 μM, ERβ at 8.8 μM; α-eleostearic acid (AEA) inhibited ERαERβ at 6.57.8 μM. PA (not AEA) agonized ERαERβ (EC50) at 1.82 μM, antagonizing at 10180 μM. AEA antagonized ERαERβ at 150140 μM. PA and AEA induced ERα and ERβ mRNA expression in MCF-7, but not in MDA-MB-231. Overall, the results show PA and AEA are SERMs.
Bibliographical noteFunding Information:
This work was supported by the Grant of Agricultural R&D Promotion center (ARPC, 2002–2005), Republic of Korea. We thank Professor Gunter Daxenbichler, Univ-Klink fur Frauenheikunde, Innsbruck, Germany for the kind gift of breast cancer cell lines.
- Breast cancer
- Conjugated linolenic acid
- Pomegranate (Punica granatum)
- Punicic acid
- Selective estrogen receptor modulator
ASJC Scopus subject areas