Plakofuranolactone as a quorum quenching agent from the Indonesian sponge plakortis cf. lita

Valeria Costantino; Gerardo Della Sala; Kumar Saurav; Roberta Teta; Rinat Bar-Shalom; Alfonso Mangoni; Laura Steindler

doi:10.3390/md15030059

Plakofuranolactone as a quorum quenching agent from the Indonesian sponge plakortis cf. lita

Valeria Costantino, Gerardo Della Sala, Kumar Saurav, Roberta Teta, Rinat Bar-Shalom, Alfonso Mangoni, Laura Steindler

Department of Marine Biology

Research output: Contribution to journal › Article › peer-review

Abstract

There is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation system that allows bacteria to communicate with each other and coordinate group behaviors. A new γ-lactone that is capable of inhibiting the LasI/R QS system, plakofuranolactone (1), was discovered in the extract of the marine sponge Plakortis cf. lita, and its structure, including absolute configuration, was determined by NMR spectroscopy, MS spectrometry, and quantum-mechanical prediction of optical rotation. The quorum quenching activity of plakofuranolactone was evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075, E. coli pSB401, and C. violeaceum CV026) and was confirmed by measuring the total protease activity (a virulence factor which is under control of the LasI/R system) of the wild-type P. aeruginosa PAO1. Further research will be pursued to assess the potential of plakofuranolactone as a new antivirulence lead compound and a chemical tool to increase the knowledge in this field.

Original language	English
Article number	59
Journal	Marine Drugs
Volume	15
Issue number	3
DOIs	https://doi.org/10.3390/md15030059
State	Published - 28 Feb 2017

Bibliographical note

Funding Information:
This work was funded by the European Union's Seventh Framework Programme (FP7) 2007-2013 under Grant Agreement No. 311848 (Bluegenics) and by the Università di Napoli Federico II under the STAR project (SeaLEADS). This study was partially supported by the Bi-lateral Italy-Israel R&D grant, MOST grant#10705-3 to LS entitled: "A novel approach to fight antibiotic-resistant pathogens: acquisition of quorum sensing inhibitors from marine sponges" (by the Ministry of Science and Technology, Israel). K.S. had a post-doctoral fellowship from the Israeli Council for Higher Education (VATAT) and University of Haifa.

Keywords

Antibacterial from marine sponges
Antivirulence lead compounds
Marine sponges
Plakofuranolactone
Plakortis lita
Pseudomonas aeruginosa
Quorum quenching activity
Quorum sensing system
Reporter gene assays

ASJC Scopus subject areas

Drug Discovery

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10.3390/md15030059

Cite this

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title = "Plakofuranolactone as a quorum quenching agent from the Indonesian sponge plakortis cf. lita",

abstract = "There is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation system that allows bacteria to communicate with each other and coordinate group behaviors. A new γ-lactone that is capable of inhibiting the LasI/R QS system, plakofuranolactone (1), was discovered in the extract of the marine sponge Plakortis cf. lita, and its structure, including absolute configuration, was determined by NMR spectroscopy, MS spectrometry, and quantum-mechanical prediction of optical rotation. The quorum quenching activity of plakofuranolactone was evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075, E. coli pSB401, and C. violeaceum CV026) and was confirmed by measuring the total protease activity (a virulence factor which is under control of the LasI/R system) of the wild-type P. aeruginosa PAO1. Further research will be pursued to assess the potential of plakofuranolactone as a new antivirulence lead compound and a chemical tool to increase the knowledge in this field.",

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author = "Valeria Costantino and Sala, {Gerardo Della} and Kumar Saurav and Roberta Teta and Rinat Bar-Shalom and Alfonso Mangoni and Laura Steindler",

note = "Funding Information: This work was funded by the European Union's Seventh Framework Programme (FP7) 2007-2013 under Grant Agreement No. 311848 (Bluegenics) and by the Universit{\`a} di Napoli Federico II under the STAR project (SeaLEADS). This study was partially supported by the Bi-lateral Italy-Israel R&D grant, MOST grant#10705-3 to LS entitled: {"}A novel approach to fight antibiotic-resistant pathogens: acquisition of quorum sensing inhibitors from marine sponges{"} (by the Ministry of Science and Technology, Israel). K.S. had a post-doctoral fellowship from the Israeli Council for Higher Education (VATAT) and University of Haifa.",

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AU - Bar-Shalom, Rinat

AU - Mangoni, Alfonso

AU - Steindler, Laura

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N2 - There is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation system that allows bacteria to communicate with each other and coordinate group behaviors. A new γ-lactone that is capable of inhibiting the LasI/R QS system, plakofuranolactone (1), was discovered in the extract of the marine sponge Plakortis cf. lita, and its structure, including absolute configuration, was determined by NMR spectroscopy, MS spectrometry, and quantum-mechanical prediction of optical rotation. The quorum quenching activity of plakofuranolactone was evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075, E. coli pSB401, and C. violeaceum CV026) and was confirmed by measuring the total protease activity (a virulence factor which is under control of the LasI/R system) of the wild-type P. aeruginosa PAO1. Further research will be pursued to assess the potential of plakofuranolactone as a new antivirulence lead compound and a chemical tool to increase the knowledge in this field.

AB - There is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation system that allows bacteria to communicate with each other and coordinate group behaviors. A new γ-lactone that is capable of inhibiting the LasI/R QS system, plakofuranolactone (1), was discovered in the extract of the marine sponge Plakortis cf. lita, and its structure, including absolute configuration, was determined by NMR spectroscopy, MS spectrometry, and quantum-mechanical prediction of optical rotation. The quorum quenching activity of plakofuranolactone was evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075, E. coli pSB401, and C. violeaceum CV026) and was confirmed by measuring the total protease activity (a virulence factor which is under control of the LasI/R system) of the wild-type P. aeruginosa PAO1. Further research will be pursued to assess the potential of plakofuranolactone as a new antivirulence lead compound and a chemical tool to increase the knowledge in this field.

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ER -

Plakofuranolactone as a quorum quenching agent from the Indonesian sponge plakortis cf. lita

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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