Placental protein 13 (PP-13): Effects on cultured trophoblasts, and its detection in human body fluids in normal and pathological pregnancies

O. Burger, E. Pick, J. Zwickel, M. Klayman, H. Meiri, R. Slotky, S. Mandel, L. Rabinovitch, Y. Paltieli, A. Admon, R. Gonen

Research output: Contribution to journalArticlepeer-review

Abstract

Placental tissue protein 13 (PP-13), one of the 56 known placental proteins identified till today, was purified from placentas obtained from women at delivery, and used to evoke antibodies against it. The purified PP-13 was lysed to peptides, which were sequenced, leading to the full-length cDNA sequencing and its expression in Escherichia coli. Sequence analysis in databases showed homology to the galectin family.Of the various antibody preparations developed, a pair of monoclonal antibodies (MAbs) coupled to the recombinant PP-13 (PP-13-R) was used for the immunodetection of PP-13 in pregnant women's serum with the solid-phase ELISA format. With a dynamic range of 25-500 pg/mL with no background in non-pregnant women's serum and men's serum, the ELISA test was suitable for the detection of PP-13 in the 1st, 2nd, and 3rd trimesters. PP-13 levels slowly increase during pregnancy. In the 1st trimester, lower than normal PP-13 levels were found in fetal growth restriction (IUGR), preeclampsia (PE), and particularly in early PE (<34 weeks of gestation). In the 2nd and 3rd trimesters, higher than normal concentrations were found in PE, IUGR and in preterm delivery (PTD).Application of PP-13 to cultured trophoblasts elicited depolarization carried by calcium ions, followed by liberation of linoleic and arachidonic acids from the trophoblast membrane, and a subsequent elevation of prostacyclin and thromboxane. These effects were negligible when PP-13 derived from the placentas of patients with IUGR, PE or PTD was used. The results are discussed in view of the potential utilization of PP-13 for early serum screening to assess the risk to develop placental insufficiency, coupled to a differential analysis of the various pathologies by analyzing cultured trophoblasts.

Original languageEnglish
Pages (from-to)608-622
Number of pages15
JournalPlacenta
Volume25
Issue number7
DOIs
StatePublished - Aug 2004
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by Research Grant DISMED 52, the German Ministry for Research and Technology (BMFT), the Israel National Council for Research and Development, Israel Ministry of Science, and the Office of the Chief Scientist, Israel Ministry of Industry and Trade (#27698 and #31851). We thank Mrs. Haia Tal for her assistance in preparing this manuscript and Dr. Ilana Maor for her assistance in the development of the cooperation with Dr. Than of the PECS University, Hungary.

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology

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