PIP 3 controls synaptic function by maintaining AMPA receptor clustering at the postsynaptic membrane

Kristin L. Arendt; María Royo; Mónica Fernández-Monreal; Shira Knafo; Cortney N. Petrok; Jeffrey R. Martens; José A. Esteban

doi:10.1038/nn.2462

PIP 3 controls synaptic function by maintaining AMPA receptor clustering at the postsynaptic membrane

Kristin L. Arendt, María Royo, Mónica Fernández-Monreal, Shira Knafo, Cortney N. Petrok, Jeffrey R. Martens, José A. Esteban

Research output: Contribution to journal › Article › peer-review

Abstract

Despite their low abundance, phosphoinositides are critical regulators of intracellular signaling and membrane compartmentalization. However, little is known of phosphoinositide function at the postsynaptic membrane. Here we show that continuous synthesis and availability of phosphatidylinositol-(3,4,5)- trisphosphate (PIP 3) at the postsynaptic terminal is necessary for sustaining synaptic function in rat hippocampal neurons. This requirement was specific for synaptic, but not extrasynaptic, AMPA receptors, nor for NMDA receptors. PIP 3 downregulation impaired PSD-95 accumulation in spines. Concomitantly, AMPA receptors became more mobile and migrated from the postsynaptic density toward the perisynaptic membrane within the spine, leading to synaptic depression. Notably, these effects were only revealed after prolonged inhibition of PIP 3 synthesis or by direct quenching of this phosphoinositide at the postsynaptic cell. Therefore, we conclude that a slow, but constant, turnover of PIP 3 at synapses is required for maintaining AMPA receptor clustering and synaptic strength under basal conditions.

Original language	English
Pages (from-to)	36-44
Number of pages	9
Journal	Nature Neuroscience
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/nn.2462
State	Published - 17 Jan 2010
Externally published	Yes

Bibliographical note

Funding Information:
We thank R. Holz (University of Michigan) for the plasmid containing the PH-PLC sequence, A. Lee (University of Michigan) for the plasmid containing the catalytic domain of mouse PI3K (p110α), C. Dotti (Katholieke Universiteit Leuven) for the membrane-anchored GFP-CAAX construct, R. Malinow (University of California, San Diego) for the SEP-GluA2 construct and L. Chen (University of California, Berkeley) for facilitating some of the experiments carried out by K.L.A. We also thank S. Jurado and members of the Esteban laboratory for critical reading of this manuscript, and S. Fisher, E. Stuenkel, G. Murphy and R. Holz for discussions. This work was supported by grants from the US National Institute of Mental Health (J.A.E. and J.R.M.), the Dana Foundation (J.A.E.) and the Spanish Ministry of Science and Innovation (J.A.E.). M.F.-M. and S.K. are supported by postdoctoral contracts, and M.R. by a predoctoral fellowship, from the Spanish Ministry of Science and Innovation.

ASJC Scopus subject areas

General Neuroscience

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title = "PIP 3 controls synaptic function by maintaining AMPA receptor clustering at the postsynaptic membrane",

abstract = "Despite their low abundance, phosphoinositides are critical regulators of intracellular signaling and membrane compartmentalization. However, little is known of phosphoinositide function at the postsynaptic membrane. Here we show that continuous synthesis and availability of phosphatidylinositol-(3,4,5)- trisphosphate (PIP 3) at the postsynaptic terminal is necessary for sustaining synaptic function in rat hippocampal neurons. This requirement was specific for synaptic, but not extrasynaptic, AMPA receptors, nor for NMDA receptors. PIP 3 downregulation impaired PSD-95 accumulation in spines. Concomitantly, AMPA receptors became more mobile and migrated from the postsynaptic density toward the perisynaptic membrane within the spine, leading to synaptic depression. Notably, these effects were only revealed after prolonged inhibition of PIP 3 synthesis or by direct quenching of this phosphoinositide at the postsynaptic cell. Therefore, we conclude that a slow, but constant, turnover of PIP 3 at synapses is required for maintaining AMPA receptor clustering and synaptic strength under basal conditions.",

author = "Arendt, {Kristin L.} and Mar{\'i}a Royo and M{\'o}nica Fern{\'a}ndez-Monreal and Shira Knafo and Petrok, {Cortney N.} and Martens, {Jeffrey R.} and Esteban, {Jos{\'e} A.}",

note = "Funding Information: We thank R. Holz (University of Michigan) for the plasmid containing the PH-PLC sequence, A. Lee (University of Michigan) for the plasmid containing the catalytic domain of mouse PI3K (p110α), C. Dotti (Katholieke Universiteit Leuven) for the membrane-anchored GFP-CAAX construct, R. Malinow (University of California, San Diego) for the SEP-GluA2 construct and L. Chen (University of California, Berkeley) for facilitating some of the experiments carried out by K.L.A. We also thank S. Jurado and members of the Esteban laboratory for critical reading of this manuscript, and S. Fisher, E. Stuenkel, G. Murphy and R. Holz for discussions. This work was supported by grants from the US National Institute of Mental Health (J.A.E. and J.R.M.), the Dana Foundation (J.A.E.) and the Spanish Ministry of Science and Innovation (J.A.E.). M.F.-M. and S.K. are supported by postdoctoral contracts, and M.R. by a predoctoral fellowship, from the Spanish Ministry of Science and Innovation.",

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AU - Petrok, Cortney N.

AU - Martens, Jeffrey R.

AU - Esteban, José A.

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PIP 3 controls synaptic function by maintaining AMPA receptor clustering at the postsynaptic membrane

Abstract

Bibliographical note

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