TY - JOUR
T1 - Phylogenetically and metabolically diverse active carbon-fixing microbes reside in mangrove sediments
AU - Wang, Shasha
AU - Zhao, Zhuoming
AU - Cheng, Ruolin
AU - Cui, Liang
AU - Wang, Jun
AU - Rubin-Blum, Maxim
AU - Zhang, Yao
AU - Liu, Bolin
AU - Chen, Xing
AU - Baltar, Federico
AU - Cao, Xiaxing
AU - Wen, Xuezhe
AU - Alain, Karine
AU - Chen, Zhen
AU - Liao, Jing
AU - Jiang, Lijing
AU - Shao, Zongze
N1 - Publisher Copyright:
© The Author(s) 2025.
PY - 2025/12
Y1 - 2025/12
N2 - Background: Mangroves are hotspots of carbon sequestration in transitional zones between marine and terrestrial ecosystems. Microbially driven dark carbon fixation (DCF) is prominent in sediments, yet our understanding of the DCF process across this continuum remains limited. In this study, we explored DCF activities and associated chemoautotrophs along the sediment depth of different mangrove sites in Fujian Province, China, using radiocarbon labeling and molecular techniques. Results: Our results showed that the DCF rates ranged from 0.02 to 3.27 mmol C m−2 day−1 in all samples, showing a depth-dependent spatial variation. These rates of DCF were closely related to the environmental factors such as DIC, TS, AVS, NH4+, NO3−, and NO2−. Metagenomic analysis revealed six carbon-fixing pathways, with the Calvin-Benson-Bassham (CBB) cycle and Wood-Ljungdahl (WL) pathway being predominant. Further analysis of MAGs revealed that Gammaproteobacteria, Desulfobacteria, and Campylobacteria were the most abundant carbon-fixing groups. Intriguingly, some new lineages were found to have carbon-fixing potential, including two candidatus taxa JAJVIF01 and BMS3Abin14. Metatranscriptomic analyses confirmed that these carbon-fixing microbes were active in situ and occupied different niches. In the surface layers, Gammaproteobacteria with the CBB cycle played an important role in DCF, mainly driven by sulfur and hydrogen oxidation with oxygen reduction; in the deeper layers, Campylobacteria with the reductive tricarboxylic acid (rTCA) cycle and Desulfobacteria with the WL pathway were active members for DCF, mainly through sulfur, hydrogen, and CO oxidation. While in the deepest layers of 18–20 cm, methane-producing archaea Methanosarcinia was the essential member driving DCF. In addition, most taxa containing the WL pathway displayed a mixotrophic lifestyle corresponding to flexible carbon acquisition strategies. Conclusions: Overall, this study provides new insights into the understanding of biological carbon fixation and its ecological functions in mangrove sediments.
AB - Background: Mangroves are hotspots of carbon sequestration in transitional zones between marine and terrestrial ecosystems. Microbially driven dark carbon fixation (DCF) is prominent in sediments, yet our understanding of the DCF process across this continuum remains limited. In this study, we explored DCF activities and associated chemoautotrophs along the sediment depth of different mangrove sites in Fujian Province, China, using radiocarbon labeling and molecular techniques. Results: Our results showed that the DCF rates ranged from 0.02 to 3.27 mmol C m−2 day−1 in all samples, showing a depth-dependent spatial variation. These rates of DCF were closely related to the environmental factors such as DIC, TS, AVS, NH4+, NO3−, and NO2−. Metagenomic analysis revealed six carbon-fixing pathways, with the Calvin-Benson-Bassham (CBB) cycle and Wood-Ljungdahl (WL) pathway being predominant. Further analysis of MAGs revealed that Gammaproteobacteria, Desulfobacteria, and Campylobacteria were the most abundant carbon-fixing groups. Intriguingly, some new lineages were found to have carbon-fixing potential, including two candidatus taxa JAJVIF01 and BMS3Abin14. Metatranscriptomic analyses confirmed that these carbon-fixing microbes were active in situ and occupied different niches. In the surface layers, Gammaproteobacteria with the CBB cycle played an important role in DCF, mainly driven by sulfur and hydrogen oxidation with oxygen reduction; in the deeper layers, Campylobacteria with the reductive tricarboxylic acid (rTCA) cycle and Desulfobacteria with the WL pathway were active members for DCF, mainly through sulfur, hydrogen, and CO oxidation. While in the deepest layers of 18–20 cm, methane-producing archaea Methanosarcinia was the essential member driving DCF. In addition, most taxa containing the WL pathway displayed a mixotrophic lifestyle corresponding to flexible carbon acquisition strategies. Conclusions: Overall, this study provides new insights into the understanding of biological carbon fixation and its ecological functions in mangrove sediments.
KW - Chemolithoautotroph
KW - Dark carbon fixation
KW - In situ
KW - Mangrove sediments
KW - Metatranscriptomics
KW - Vertical distribution
UR - https://www.scopus.com/pages/publications/105014923259
U2 - 10.1186/s40168-025-02177-9
DO - 10.1186/s40168-025-02177-9
M3 - Article
C2 - 40890888
AN - SCOPUS:105014923259
SN - 2049-2618
VL - 13
JO - Microbiome
JF - Microbiome
IS - 1
M1 - 194
ER -