Phosphorylation of JDP2 on threonine-148 by the c-Jun N-terminal kinase targets it for proteosomal degradation

Keren Weidenfeld-Baranboim, Lilach Koren, Ami Aronheim

Research output: Contribution to journalArticlepeer-review

Abstract

JDP2 (c-Jun dimerization protein 2) is a member of the basic leucine zipper family of transcription factors that is ubiquitously expressed in all examined cell types. JDP2 is phosphorylated on Thr148 by JNK (c-Jun N-terminal kinase) and p38 kinase, although the functional role of its phosphorylation is unknown. In the present paper we show that the JDP2 protein level is dramatically reduced in response to serum stimulation, anisomycin treatment, ultraviolet light irradiation and cycloheximide treatment, all of which activate the JNK pathway. In addition, endogenous and overexpressed JDP2 are phosphorylated in response to these stimuli. Replacement of Thr148 with an alanine residue stabilizes ectopically expressed JDP2 in the presence of the stimuli; conversely, substitution with glutamic acid destabilizes it. Serum-induced phosphorylation and degradation of JDP2 are specific to JNK activation since a JNK inhibitor (SP600125) abolishes these effects, whereas p38 and MEK inhibitors (SB203580 and UO126) have no effect. In the presence of cycloheximide, JDP2 is rapidly phosphorylated and degraded due to the combined effects of protein synthesis inhibition and activation of JNK. Pretreatment of cells with SP600125 prior to cycloheximide treatment significantly prolongs the half-life of JDP2 that is found mainly in the unphosphorylated form. Lastly, the proteasome inhibitor (MG132) rescues JDP2 degradation following cycloheximide treatment and increases the expression of the JDP2 phosphomimetic T148E mutant. Collectively, these results suggest that phosphorylation of JDP2 on thr 148 by JNK targets it to the proteasome for degradation.

Original languageEnglish
Pages (from-to)661-669
Number of pages9
JournalBiochemical Journal
Volume436
Issue number3
DOIs
StatePublished - 15 Jun 2011
Externally publishedYes

Keywords

  • Activator protein 1 (AP-1)
  • c-Jun
  • c-Jun dimerization protein 2 (JDP2)
  • c-Jun N-terminal kinase
  • Phosphorylation
  • Protein stability

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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