Pharmacological and Immunohistochemical Evidence for Serotonergic Modulation of Cholinergic Nucleus Basalis Neurons

A. Khateb; P. Fort; A. Alonso; B. E. Jones; M. Mülethaler

doi:10.1111/j.1460-9568.1993.tb00519.x

Pharmacological and Immunohistochemical Evidence for Serotonergic Modulation of Cholinergic Nucleus Basalis Neurons

A. Khateb, P. Fort, A. Alonso, B. E. Jones, M. Mülethaler

Research output: Contribution to journal › Article › peer-review

Abstract

Identified electrophysiologically by low threshold bursts and transient outward rectification, cholinergic nucleus basalis neurons were recorded and labelled intracellularly in guinea‐pig basal forebrain slices. By means of a triple labelling immunofluorescent technique, serotonin‐immunoreactive fibres were visualized in close proximity to the soma and dendrites of the biocytin‐labelled, choline acetyl transferase (ChAT)‐immunoreactive cells. By bath application, 5‐hydroxytryptamine (5‐HT) produced a direct hyperpolarization of the identified cells which was mimicked by 5‐HT_1A receptor agonists, suggesting that it may inhibit the tonic firing but also modulate the low threshold bursting of the cholinergic nucleus basalis neurons.

Original language	English
Pages (from-to)	541-547
Number of pages	7
Journal	European Journal of Neuroscience
Volume	5
Issue number	5
DOIs	https://doi.org/10.1111/j.1460-9568.1993.tb00519.x
State	Published - May 1993
Externally published	Yes

Keywords

5‐HT receptor
guinea‐pig
low threshold spike
sleep
substantia innominata

ASJC Scopus subject areas

Neuroscience (all)

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10.1111/j.1460-9568.1993.tb00519.x

Cite this

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title = "Pharmacological and Immunohistochemical Evidence for Serotonergic Modulation of Cholinergic Nucleus Basalis Neurons",

abstract = "Identified electrophysiologically by low threshold bursts and transient outward rectification, cholinergic nucleus basalis neurons were recorded and labelled intracellularly in guinea‐pig basal forebrain slices. By means of a triple labelling immunofluorescent technique, serotonin‐immunoreactive fibres were visualized in close proximity to the soma and dendrites of the biocytin‐labelled, choline acetyl transferase (ChAT)‐immunoreactive cells. By bath application, 5‐hydroxytryptamine (5‐HT) produced a direct hyperpolarization of the identified cells which was mimicked by 5‐HT1A receptor agonists, suggesting that it may inhibit the tonic firing but also modulate the low threshold bursting of the cholinergic nucleus basalis neurons.",

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AU - Khateb, A.

AU - Fort, P.

AU - Alonso, A.

AU - Jones, B. E.

AU - Mülethaler, M.

PY - 1993/5

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N2 - Identified electrophysiologically by low threshold bursts and transient outward rectification, cholinergic nucleus basalis neurons were recorded and labelled intracellularly in guinea‐pig basal forebrain slices. By means of a triple labelling immunofluorescent technique, serotonin‐immunoreactive fibres were visualized in close proximity to the soma and dendrites of the biocytin‐labelled, choline acetyl transferase (ChAT)‐immunoreactive cells. By bath application, 5‐hydroxytryptamine (5‐HT) produced a direct hyperpolarization of the identified cells which was mimicked by 5‐HT1A receptor agonists, suggesting that it may inhibit the tonic firing but also modulate the low threshold bursting of the cholinergic nucleus basalis neurons.

AB - Identified electrophysiologically by low threshold bursts and transient outward rectification, cholinergic nucleus basalis neurons were recorded and labelled intracellularly in guinea‐pig basal forebrain slices. By means of a triple labelling immunofluorescent technique, serotonin‐immunoreactive fibres were visualized in close proximity to the soma and dendrites of the biocytin‐labelled, choline acetyl transferase (ChAT)‐immunoreactive cells. By bath application, 5‐hydroxytryptamine (5‐HT) produced a direct hyperpolarization of the identified cells which was mimicked by 5‐HT1A receptor agonists, suggesting that it may inhibit the tonic firing but also modulate the low threshold bursting of the cholinergic nucleus basalis neurons.

KW - 5‐HT receptor

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Pharmacological and Immunohistochemical Evidence for Serotonergic Modulation of Cholinergic Nucleus Basalis Neurons

Abstract

Keywords

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